Gastroprotective Effectiveness Test of 70% Ethanol Extract of Karamunting Leaves on White Male Rats (Rattus norvegicus)

Saru Noliqo Rangkuti; Nuraini; Melisa

doi:10.35451/1w27kw61

Authors

Saru Noliqo Rangkuti Fakultas Farmasi, Universitas Muhammadiyah A.R Fachruddin
Nuraini Fakultas Farmasi, Universitas Muhammadiyah A.R Fachruddin
Melisa Fakultas Farmasi, Universitas Muhammadiyah A.R Fachruddin

DOI:

https://doi.org/10.35451/1w27kw61

Keywords:

Karamunting Leaf, Gastroprotective, Male rats

Abstract

Background: Gastritis is a health disorder in digestion, especially the stomach. Treatment of gastritis can be cured by using medicinal plants which are thought to have compounds that are efficacious as gastroprotective. The karamunting plant is one of the biodiversity that must be developed because the karamunting plant has one of the properties as a gastroprotective. Karamunting, among others, has various kinds of ingredients such as flavonoids, saponins, tannins, alkaloids, and triterpenoids which are useful as herbal medicines. Objective: This study aims to determine the content of secondary metabolites, determine the effectiveness of healing gastritis in rats and determine what dose of 70% ethanol extract of karamunting leaves has the effectiveness of gastroprotective healing in rats. Method: Test animals were divided into 6 groups consisting of group I negative control CMC 0.5%; group II positive control of 500mg/5ml sucralfate; group III induction control 1000mg/kgBB; groups IV, V, VI 70% ethanol extract of karamunting leaves at a dose of 20mg/200g BW of rats, a dose of 40mg/200g BW of rats and a dose of 60mg/200g BW of rats. After the treatment, the animals were dissected and the stomach was observed macroscopically. Result: Data were analyzed with the Kruskal Wallis test, and followed by the Mann-Whitney test. The results of this study showed that 70% ethanol extract of karamunting leaves has effectiveness as a gastroprotective. A dose of 20mg/200gBW rats has a % protection value of 76%, a dose of 40mg/200gBW rats has a % protection value of 75%, and a dose of 60mg/200gBW rats has a % protection value of 100%. Statistical test results showed that there was a significant difference in each group on gastroprotective effectiveness (p <0.05). Conclusion: The conclusion of this study was that the 70% ethanol extract of caramunting leaves had the most optimal gastroprotective effectiveness at dose III, namely 60mg/200gBW rats.

Downloads

Download data is not yet available.

References

[1] NABILA, C., 2024. GAMBARAN HISTOPATOLOGI TOKSISITAS HEPAR TIKUS (Rattus norvegicus) BETINA PASCA-PEMBERIAN EKSTRAK ETANOL DAUN KARAMUNTING (Rhodomyrtus tomentosa) (Doctoral dissertation, Kedokteran).

[2] Siregar, E.D.N.F., Oktiani, B.W., Kurniawan, F.K.D., Aspriyanto, D. and Wardani, I.K., 2024. UJI TOKSISITAS EKSTRAK DAUN KARAMUNTING (Rhodomyrtus tomentosa (Aiton) Hassk.) TERHADAP HATI TIKUS WISTAR. Dentin, 8(2).

[3] Wibowo, M.R., Oktiani, B.W., Budipramana, M., Apriasari, M.L. and Firdaus, I.W.A.K., 2024. UJI TOKSISITAS EKSTRAK DAUN KARAMUNTING (Rhodomyrtus tomentosa (Aiton) Hassk.) TERHADAP GINJAL TIKUS WISTAR (Berdasarkan Ureum dan Kreatinin). Dentin, 8(2).

[4] Anggraeni, L.N., Fakhruddin, F. and Irawan, Y., 2021. Pengaruh Pemberian Ekstrak Etanol Daun Karamunting (Rhodomyrtus Tomentosa (Ait.) Hassk.) terhadap Kadar Kolesterol dan Trigliserida pada Mencit Putih Hiperlipidemia. Jurnal Kesehatan Borneo Cendekia, 5(1), pp.96-104.

[5] Fadilah, N.A., Enis, R.N. and Ekaputri, T.W., 2025. Uji Efektivitas Ekstrak Daun Karamunting (Rhodomyrtus tomentosa) sebagai Nefroprotektif pada Tikus (Rattus norvegicus) Hiperkolesterolemia (Doctoral dissertation, Universitas Jambi).

[6] Pusmarani, J. and Putri, R.J., 2022. Aktivitas Gastroprotektif Ekstrak Metanol Kulit Semangka (Citrullus lanatus L.) Pada Tikus (Rattus norvegicus) Yang Diinduksi Aspirin. Jurnal Pharmacia Mandala Waluya, 1(1), pp.21-36.

[7] Marbun, R.A.T. and Sitorus, P., 2025. Immunomodulatory effect of Artemisia vulgaris L. ethanol extract and its marker compound on antibody and cytokine release in non-immunosuppressed and immunosuppressed rats. Tropical Journal of Natural Product Research, 9(3).

[8] Sihombing, Y. R., Marbun, R. A. T., S, A. and Siska, F. (2024) “Effect of guazuma ulmifolia lamk. extract on interleukin-1 and interleukin-2 levels as immunomodulatory agent candidates ”, Science Midwifery, 12(4), pp. 1493-1499. doi: 10.35335/midwifery.v12i4.1714.

[9] Situmorang, N.B., Dakhi, J.V. and Marbun, R.A.T., 2022. Aktivitas Antihiperurisemia Ekstrak Etanol Biji Mahoni (Swietenia macrophylla) Terhadap Tikus Jantan. Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice), 9(1), pp.12-16.

[10] Windari, T., 2017. Peranan ekstrak bawang dayak (Eleutherine palmifolia) sebagai agen anti tukak lambung (peptic ulcer) pada Tikus Wistar (Rattus norvegicus) jantan yang diinduksi etanol. Jurnal Pangan dan Agroindustri, 5(1).

[11] Putri, C.A., Ramadani, A.P. and Maulida, F.R., 2019. Efek Gastroprotektif Ekstrak Etanol Daun Pepaya (Carica papaya L.) pada Tikus Jantan yang Diinduksi Aspirin. EKSAKTA: Journal of Sciences and Data Analysis, pp.98-104.

[12] Rizqonnisa, A., 2023. Uji Aktivitas Gastroprotektif Infusa Rimpang Temu Putih (Curcuma zedoaria (Christm.) Roscoe) Terhadap Tikus Putih Jantan yang di Induksi Etanol.

[13] Ramadhani, S.E., 2024. PENGARUH EKSTRAK KAYU MANIS (Cinnamomum Burmannii) TERHADAP INDEKS ULKUS GASTER Studi Eksperimental pada Tikus Jantan Galur Wistar yang Diinduksi Ibuprofen (Doctoral dissertation, Universitas Islam Sultan Agung Semarang).

[14] Padmasari, P.D., Astuti, K.W. and Warditiani, N.K., 2013. Skrining fitokimia ekstrak etanol 70% rimpang bangle (Zingiber purpureum Roxb.). Jurnal Farmasi Udayana, 2(4), p.279764.

[15] Rupina, W., Trianto, H.F. and Fitrianingrum, I., 2016. Efek Salep Ekstrak Etanol 70% Daun Karamunting terhadap Re-epitelisasi Luka Insisi Kulit Tikus Wistar. FK Tanjungpura.

[16] Wistar, T.P.J.G., Efek Ekstrak Etanol 70% Daun Karamunting (Rhodomyrtus tomentos (Ait.) Hassk) Topikal terhadap Pertumbuhan Jumlah Sel Fibroblas Luka Insisi Kulit.

[17] Khatimah, H., 2023. Pengaruh Ekstrak Temu Putih (Curcuma Zedoaria) Dengan Dosis Bertingkat Terhadap Hepar Mencit (Mus Musculus) Jantan Yang Diinduksi Aspirin (Doctoral dissertation, Universitas Islam Negeri Ar-Raniry).

[18] Wibowo, M.R., Oktiani, B.W., Budipramana, M., Apriasari, M.L. and Firdaus, I.W.A.K., 2024. Uji Toksisitas Ekstrak Daun Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk.) TERHADAP GINJAL Tikus Wistar (Berdasarkan Ureum dan Kreatinin). Dentin, 8(2).

[19] Rosa, F.L., 2023, November. Studi pendekatan molekuler modeling senyawa golongan flavonoid sebagai antiulcer pada tukak lambung. In lambung mangkurat medical seminar (Vol. 4, No. 1, pp. 605-622).

[20] NOFIANTI, T., 2024. The Potency Mucoadhesive Granules of Ethanol Extract Green Grass Jelly (Premna oblongifolia MERR.) as Peptic Ulcer Treatment Agent. Indonesian Journal of Pharmaceutical Science and Technology, pp.174-182.