Antibacterial Activity of Cananga Extract (Cananga odorata (Lam.) Hook.F. & Thomson) Against Streptococcus Mutans and Pseudomonas Aeruginosa Bacteria
DOI:
https://doi.org/10.35451/nyc2kg76Keywords:
Cananga flower, Antibacterial, Streptococcus mutans, Pseudomonas aeruginosa, Disc diffusionAbstract
Background: Streptococcus mutans is a gram-positive bacterium commonly found in the oral cavity, while Pseudomonas aeruginosa is a gram-negative rod-shaped bacterium that can form biofilms. Objective: The objective of this study was to determine the antibacterial activity of 96% ethanol extract of ylang-ylang flowers (Cananga odorata (Lam.) Hook.f. & Thomson) against S. mutans and P. aeruginosa using the disk diffusion method. Methods: This was an experimental study with a post-test only control group design. Results: The test results showed that ylang-ylang flower extract exhibited antibacterial activity against S. mutans with an average zone at a 40% concentration of 16.6 ± 2.5 mm, categorized as strong. Meanwhile, against P. aeruginosa, the average inhibition zone at a 40% concentration was 7.2 ± 2.2 mm, categorized as moderate. Phytochemical analysis showed that kenanga flower extract contains flavonoids, saponins, tannins, steroids, monoterpenes, and alkaloids. Conclusion: 96% ethanol extract of kenanga flowers effectively inhibits the growth of S. mutans and P. aeruginosa at a concentration of 40%. These results indicate that kenanga has potential as a natural antibacterial source for development in the pharmaceutical field.
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[1] Puluhulawa LE, Paneo MA. Peningkatan Pemahaman Masyarakat Mengenai Penyakit Akibat Infeksi Di Puskesmas Kota Timur Gorontalo. Jurnal Pengabdian Masyarakat Farmasi: Pharmacare Society[Online]. 2024;3(1):1–6. https://doi.org/10.37905/phar.soc.v3i1.24944
[2] Wigunarti AH, Pujiyanto S, Suprihadi A. Uji Aktivitas Antibakteri Ekstrak Biji Kelor (Moringa oleifera L.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Bakteri Escherichia coli. Berkala Bioteknologi [Online]. 2019 Dec;2(2). Retrieved from: https://ejournal2.undip.ac.id/index.php/bb/article/view/6712.
[3] Katrin D, Idiawati N, Sitorus B. Uji Aktivitas Antibakteri Dari Ekstrak Daun Malek (Litsea graciae vidal) Terhadap Bakteri Stapylococcus aureus Dan Escherichia coli. Jurnal Kimia Khatulistiwa [Online]. 2015;4(1):7–12. Retrieved from: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/11720.
[4] Herdini, Bahri S, Natasya Y. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Brotowali (Tinospora cordifolia (Willd.) Miers Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. Sainstech : Jurnal Penelitian dan Pengkajian Sains dan Teknologi [Online]. 2024;34(2):31–38. https://doi.org/10.37277/stch.v34i2.2040
[5] Endriani E, Siregar FM, Rafni E, Kemal RA, Jefrizal. Identifikasi gen kariogenik glukosiltransferase Streptococcus mutans pada pasien karies gigi. Jurnal Kedokteran Gigi Universitas Padjadjaran [Online]. 2021;33(1):14-18. https://doi.org/10.24198/jkg.v33i1.30397
[6] Wahyudi D, Soetarto ES. Pembentukan Biofilm Pseudomonas aeruginosa pada Beberapa Media Cair. Journal of Pharmacy [Online]. 2021;10(2):35-40. Retrieved from: https://www.researchgate.net/publication/356925712
[7] Nufus LS, Pertiwi D. Tingkat Pengetahuan Masyarakat Terhadap Penggunaan Antibiotik (Amoxicilin) Berdasarkan Usia Di Dusun Karang Panasan Kabupaten Lombok Utara [Online]. 2019;12(2):9. Available from: https://e-journal.lppmdianhusada.ac.id/index.php/jk/article/view/92
[8] Moningka KC, Kojong NS, Sudewi S. Uji Aktivitas Antibakteri Ekstrak Daun Ekor Kucing (Acalypha hispida burm. F.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli secara in-vitro [Online]. Pharmacon [Online]. 2015;4(3):193–202. https://doi.org/10.35799/pha.4.2015.8859
[9] Dusturia N, Hikamah SR, Sudiarti D. Efektivitas Antibakteri Bunga Kenanga (Cananga odorata) Dengan Metode Konvensional Terhadap Pertumbuhan Staphylococcus aureus. Jurnal Bioshell [Online]. 2016;5(1):324–332. Retrieved from https://ejurnal.uij.ac.id/index.php/BIO/article/view/53
[10] Semadhi PGM, Mahardika KIK, Megayanthi RS, Kirana NWP, Palaguna IDGBP, Hendrayana MA. Uji Aktivitas Antibakteri Ekstrak Kulit Batang Tanaman Kenanga (Cananga odorata) Terhadap Bakteri Penyebab Infeksi Kulit Staphylococcus aureus In Vitro. Intisari Sains Medis [Online]. 2022;13(1):6–10. https://doi.org/10.15562/ism.v13i1.1201
[11] Fajri N, Putri LFA, Prasetio MR, Azizah N, Pratama Y, Susanto NCA. Potensi Batang Pisang (Musa paradisiaca l) Sebagai Bioreduktor Dalam Green Sintesis Ag Nanopartikel. Jurnal Penelitian Sains [Online]. 2022;24(1):33-37. https://doi.org/10.56064/jps.v24i1.668
[12] Nurhayati LS, Yahdiyani N, Hidayatulloh A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan [Online]. 2020;1(2):41-46. https://doi.org/10.24198/jthp.v1i2.27537
[13] Adani MAR. Studi In Vivo : Efektivitas Kombucha Bunga Kenanga (Cananga odorata) Dan Ekstrak Kulit Apel Manalagi (Malus sylvestris) Sebagai Minuman Probiotik Anti Hiper-urisemia, Skripsi [Online]. 2024.
[14] Hita IPGAP, Arimbawa PE, Suryaningsih NPA. Characterization And Screening Active Phytochemical Compounds Of 70% Ethanol Extract Of Mahogany Seed (Swietenia mahagoni Jacq.). Ad-dawaa’ Journal Of Pharmaceutical Sciences [Online]. 2021;4(1):1-8. https://doi.org/10.24252/djps.v4i1.21225
[15] Hita IPGAP, Setiawan PYB, Septiari IG, Putra IGNAWW. Uji Aktivitas Antibakteri Ekstrak Etanol 96% Daun Sambiloto (Andrographis paniculata (Burm.f.) Nees Terhadap Propionibacterium acnes. Medfarm: Jurnal Farmasi dan Kesehatan [Online]. 2022;11(1):115–126. https://doi.org/10.48191/medfarm.v11i1.76
[16] Cahyani NPSE, Susiarni J, Dewi KCS, Melyandari NLP, Putra KWA, Swastini DA. Karakteristik Dan Skrining Fitokimia Ekstrak Etanol 70% Batang Kepuh (Sterculia foetida L.). Jurnal Kimia (Journal of Chemistry) [Online]. 2019;13(1):22. http://dx.doi.org/10.24843/JCHEM.2019.v13.i01.p04
[17] Putri AM, Muham AO, Anggraini S, Maisarmah S, Yulis PAR. Analisis Kualitatif Kandungan Bunga Kenanga (Cananga Odorata) Secara Fitokimia Dengan Menggunakan Pelarut Etanol. Journal of Research and Education Chemistry [Online]. 2020;2(1):43-48. https://doi.org/10.25299/jrec.2020.vol2(1).4783
[18] Mewengkang TT, Lintang RA, Losung F, Sumilat DA, Lumingas LJL. Identification Of Bioactive Compounds And Antibacterial Activity Of Sea Cucumber, Holothuria (Halodeima) Atra Jaeger 1833 Flesh Extract From Kalasey Coastal Waters, Minahasa District. Jurnal Ilmiah Platax [Online]. 2022;10(2):355-363. https://doi.org/10.35800/jip.v10i2.42271
[19] Abadia R. Uji Antibakteri Nanopartikel Perak (AgNPs) Ekstrak Daun Sirih Hijau (Piper betle L.) Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa Secara In Vitro. Skripsi [Online]. 2025.
[20] Fakhruzy, Kasim A, Asben A, Anwar A, Review : Optimalisasi Metode Maserasi Untuk Ekstraksi Tanin Rendemen Tinggi. Menara Ilmu [Online]. 2020;14(02):38-41. https://doi.org/10.31869/mi.v14i2.1739.
[21] Wendersteyt NV, Wewengkang DS, Abdullah SS. Antimicrobial Activity Test of Exstracts and Fractions of Ascidian Herdmania Momus From Bangka Island Waters Likupang Against the Growth of Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. Pharmacon- Program Studi Farmasi, FMIPA, Universitas Sam Ratulangi [Online]. 2021;10(1):706-712. https://doi.org/10.35799/pha.10.2021.32758
[22] Muawanah M, Nurhidayat N, Rasyid NQ, Susanti S. Analisis Kadar Siklamat Pada Selai Tidak Bermerek Yang Dijual Di Pasar Tradisional Kota Makassar. Journal of Health Science & Technology [Online]. 2020;1(2):65-72. https://doi.org/10.53861/lontarariset.v1i2.72
[23] Fatimawali, Kepel BJ, Bodhi W. Standarisasi Parameter Spesifik Dan Non-Spesifik Ekstrak Rimpang Lengkuas Merah (Alpinia Purpurata K. Schum) Sebagai Obat Antibakteri. eBiomedik [Online]. 2020; 8(1): 63–67. https://doi.org/10.35790/ebm.v8i1.28131
[24] Agustien GS, Norfriyaldi A, Yani A. Uji Mutu Ekstrak Etanol Daun Pala Muda Dan Daun Tua (Myristica fragans). Perjuangan Nature Pharmaceutical Conference [Online]. 2024;1(1):64-71. Retrieved from: https://e-journal.unper.ac.id/index.php/pnpc/article/view/1408/946
[25] Depkes RI. Farmakope Herbal Indonesia Edisi I. Departemen Kesehatan Republik Indonesia. 2008: 1–184.
[26] Muthmainnah B. Skrining Fitokimia Senyawa Metabolit Sekunder Dari Ekstrak Etanol Buah Delima (Punica Granatum L.) Dengan Metode Uji Warna. Media farmasi. 2017;13(2). Retrieved from: https://scispace.com/pdf/skrining-fitokimia-senyawa-metabolit-sekunder-dari-ekstrak-33u3g2mb76.pdf
[27] Agung SFM, Sudirga SK, Darmadi AAK. Efektivitas Ekstrak Bunga Kenanga (Cananga Odorata (Lam).) Untuk Menghambat Pertumbuhan Jamur (Colletotrichum acutatum (Jenkins & Winstead) (Penyebab Penyakit Antraknosa Pada Tanaman Cabai Besar (Capsicum annum L.). Simbiosis Program Studi Biologi FMIPA UNUD [Online]. 2022;1(2):109–121. https://doi.org/10.24843/JSIMBIOSIS.2022.v10.i01.p10
[28] Gamas CG, Dellima BREM, Putri MK. Formulasi Dan Uji Antibakteri Sabun Cair Ekstrak Etanol Bunga Kenanga (Cananga Odorata) Dengan Scrub Kulit Jeruk Keprok (Citrus Reticula Blanco.) Terhadap Staphylococcus Aureus. Jurnal Ilmu Kesehatan (JIKA) [Online]. 2023;2(2):34–49. Retrieved from: https://www.ejournal.akbidyo.ac.id/index.php/JIKA/article/view/66
[29] Burhan AH, Bintoro DW, Mardiyaningsih A, Nurhaeni F. Studi Literatur: Aktivitas Antibakteri Minyak Atsiri Daun dan Batang Tanaman terhadap Bakteri Klebsiella Pneumoniae. Action Research Literate [Online]. 2022;6(2):118–33. https://doi.org/10.46799/arl.v6i2.126
[30] Asni NK, Sianita MM. Pengaruh Jumlah Crosslinker Terhadap Persen Ekstraksi Pada Sintesis Molecularly Imprinted Polymer Sebagai Adsorben Untuk Kloramfenikol The Effect of Crosslinker Amount to The Percent Extraction In Molecularly Imprinted Polymer Synthesis as An Adsorben. Unesa Journal of Chemistry [Online]. 2020;9(3):179–88. https://doi.org/10.26740/ujc.v9n3.p179-188
[31] Henaulu AH, Kaihena M. Potensi Antibakteri Ekstrak Etanol Daun Kecipir (Psophocarpus tetragonolobus (L.) DC) Terhadap Pertumbuhan Escherichia coli dan Staphylococcus aureus In Vitro. Biofaal Journal [Online]. 2020;1(1):44–54. https://doi.org/10.30598/biofaal.v1i1pp44-54
[32] Rahmawati D, Juhairiyah, Dewi SP, Fatmawati L, Mahsusiyati, Amaniya L, Madany AAE, Noroin MWE, Eka V, Fauziyah A. Efektivitas Dan Efisiensi Metode Sterilisasi Autoklaf Dalam Bidang Farmasi: Literature Review Article. Jurnal Penelitian Medis Berkelanjutan [Online]. 2025;9(2):26-33. Retrieved from: https://www.m3yapindo.com/jurnal/1805/articles/67891
[33] Nadlif AM, Walid M. Daya Hambat Staphylococcus Aureus Terhadap Ekstrak Metanol Daun Sirsak (Annona Muricata L.). OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan [Onine]. 2024;2(3):76–86. https://doi.org/10.61132/obat.v2i3.365
[34] Rosmania R, Yanti F. Perhitungan Jumlah Bakteri Di Laboratorium Mikrobiologi Menggunakan Pengembangan Metode Spektrofotometri. Jurnal Penelitian Sains [Online]. 2020;22(2):76-86. https://doi.org/10.56064/jps.v22i2.564
[35] Azizah M, Madona AA, Munarsih E. Uji Aktivitas Antibakteri Ekstrak Etanol Pelepah Pisang Ambon (Musa x paradisiaca L.) Terhadap Tiga Bakteri Penyebab Diare. Jurnal ‘Aisyiyah Medika [Online]. 2023;8(2):187-193. Retrieved from: http://jurnal.stikes-aisyiyah-palembang.ac.id/index.php/JAM/article/view/1100
[36] Dyartha AA, Lesmana D, Onggowidjaja P. Daya Antibakteri Minyak Atsiri Cananga Odorata Terhadap Pertumbuhan Bakteri Streptococcus Mutans ATCC 25175. Stomatognatic (J.K.G Unej) [Online]. 2023;20(2):103-07. https://doi.org/10.19184/stoma.v20i2.44007
[37] Muna R. Karakterisasi dan Aktivitas Bakteri Endofit Daun Kenanga (Cananga odorata) Terhadap Bakteri Staphylococcus epidermidis dan Pseudomonas aeruginosa. Skripsi [Online]. 2024.
[38] Saptowo A, Supriningrum R, Supomo. Uji Aktivitas Antibakteri Ekstrak Kulit Batang Sekilang (Embeliaborneensis scheff) Terhadap Bakteri Propionibacterium acnes dan Staphylococcus epidermidis. Al Ulum Sains dan Teknologi [Online]. 2022;7(2):93–97. https://dx.doi.org/10.31602/ajst.v7i2.6331
[39] Nurmaulawati R, Andani Y. Uji Antibakteri Ekstrak Buah Ranti Hijau (Solanum nigrum L) Terhadap Escherichia coli dan Staphylococcus aureus. PIPK [Online]. 2024;3(3):119-27. https://doi.org/10.56586/pipk.v3i3.356
[40] Fitri I, Susilowati DT, Rohmah IN. Uji Aktivitas Antibakteri Ekstrak Bonggol Pisang Kepok (Musa paradisaca Linn. var. kepok) Terhadap Staphylococcus aureus. Eduproxima: Jurnal Ilmiah Pendidikan IPA [Online]. 2021;3(1):24-30. https://doi.org/10.29100/eduproxima.v3i1.1850
[41] Sarijowan TPD, Bodhi W, Lebang JS. Uji Aktivitas Antibakteri Ekstrak Daun Afrika Terhadap Pertumbuhan Bakteri Staphylococcus aureus Dan Pseudomonas aeruginosa. Pharmacon- Program Studi Farmasi, FMIPA, Universitas Sam Ratulangi [Online]. 2022;11(4):1746–1753. https://doi.org/10.35799/pha.11.2022.42034
[42] Dewi SR, Hanifa DNC. Karakterisasi dan Aktivitas Antibakteri Minyak Serai Wangi (Cymbopogon nardus (L.) Rendle) terhadap Propionibacterium acnes. Pharm. : j. farm. Indones. [Online]. 2021;18(2):371-9. https://doi.org/10.30595/pharmacy.v18i2.7564
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