Determination of Flavonoid Content of Ethanol Extract of Yellow Pumpkin Leaves (Cucurbita moschata Dulchesne ) Using Uv-Vis Spectrophotometry

Dewi Gulyla Hari; Tri Nova Lovena; Ummul Khairiah

doi:10.35451/3rps7m67

Authors

Dewi Gulyla Hari Universitas Muhammadiyah Riau
Tri Nova Lovena Universitas Muhammadiyah Riau
Ummul Khairiah Universitas Muhammadiyah Riau

DOI:

https://doi.org/10.35451/3rps7m67

Keywords:

Pumpkin Leaves, UV-Vis Spectrophotometry, Flavonoids

Abstract

Background: Flavonoids are one of the most abundant groups of secondary metabolite compounds found in plant tissues. Pumpkin leaves (Cucurbita moschata Dulchesne ) can generally be used as antidiabetic, analgesic, nephroprotective and anticancer for stomach pain, jaundice, skin infections, lowering cholesterol, and blood sugar levels, and contain flavonoid compounds. Purpose: to determine the levels of flavonoid compounds contained in 70% ethanol extract of pumpkin leaves (Cucurbita moschata Dulchesne ). Methods: using the UV-Vis spectrophotometry method and to determine the flavonoid content from the results of phytochemical screening in the ethanol extract of pumpkin leaves. Pumpkin leaf extract was obtained through maceration techniques with 70% ethanol as the solvent. Results: The study showed that there was flavonoid content in pumpkin leaves, which was orange in color and the results of the study determined the flavonoid levels using UV-Vis spectrophotometry at a wavelength of 428 nm. om the resultsobtained from the measurement of the standard curve of quarcetin, it can be concluded that the higher the concentration used, the higher the absorbance value obtained and it can be seen that the results obtained total flavonoid content in the 70% ethanol extract sample of pumpkin leaves is 7.2672 mgQE/g. Conclusion: There is a flavonoid compound content contained in pumpkin leaves.

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References

[1] Pharmacia, J., Waluya, M., Pharmacia, J., Waluya, M., No, V., Isrul, M., Salzabillah, N. S., Lolok, N., Isrul, M., Farmasi, P. S., & Sains, F. (2023). Identifikasi Kandungan Senyawa Ekstrak Etanol Daun Labu Kuning (Cucurbita moschata ) Dan Aktivitas Antidiabetes Pada Hewan Uji Mencit (Mus musculus ) Identification of Chemical Compounds of Ethanol Extract ofYellow Pumpkin Leaves ( Cucurbita moschata ). 2(2)

[2] BPOM. (2019). Badan pengawas obat dan makanan republik indonesia Nomor 32 Tahun 2019 Tentang Persyaratan keamanan dan mutu Obat Tradisional. Bpom Ri, 11, 1–16

[3] Alza, Y., Novita, L., & Zahtamal, Z. (2023). Identifikasi Nilai Gizi Makro dan Mikro Tepung Labu Kuning Khas Riau. Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton, 9(1), 249–259.

[4] Salehi, B., Capanoglu, E., Adrar, N., Catalkaya, G., Shaheen, S., Jaffer, M., Giri, L., Suyal, R., Jugran, A. K., Calina, D., Docea, A. O., Kamiloglu, S., Kregiel, D., Antolak, H., Pawlikowska, E., Sen, S., Acharya, K., Selamoglu, Z., Sharifi-Rad, J., … Capasso, R. (2019). Cucurbits plants: A key emphasis to itspharmacological potential. Molecules, 24(10), 1–23.

[5] Naspiah, N., Pratama, M. R. F., & Sukardiman. (2021). Xanthine oxidase inhibition activity and ADMET properties of terap (Artocarpus odoratissimus Blanco) leaves metabolites: Phytochemical screening and in silico studies. Pharmacognosy Journal, 13(5), 1150–1160.

[6] Sandhya Suresh, & S S Sisodia. (2018). Phytochemical and Pharmacological Aspects of Cucurbita moschata and Moringa oleifera. Pharmaceutical and Biosciences Journal, 6(6), 45–53.

[7] Susmawati. (2021). Potensi Antidiabetes Beberapa Ekstrak Tanaman dengan Metode Induksi Aloksan

[8] Emma.J. (2023). No. In S. N. Roselina (Ed.), Buku Referensi Flavonoid.

[9] Razoki, Butar-Butar, R. G. S., Neswita, E., Sembiring, N. B., Novriani, E., Simanjuntak, N. J. P., & Pakpahan, E. H. (2023). Uji skrining fitokimia dan pengukuran kadar total flavonoid pada ekstrak paku (Nephrolepis biserrata) dengan fraksi n-heksana, etil asetat, dan air. Journal of Pharmaceutical and Sciences, 6(3), 1142–1160.

[10] Muhammad. (2023). Uji fitokimia pada daun kesum (polygonum minus huds.) Dari kalimantan barat. 5(1), 35–42.

[11] Mawardika, H., Agustina, L., & Vanesha, O. R. (2023). Uji Daya Hambat Ekstrak Etanol dan N-Heksana Daun Labu Kuning (Cucurbita moschata D.) terhadap Bakteri Staphylococcus epidermidis. Bioscientist : Jurnal Ilmiah Biologi,

[12] Purba N, Sinurat JP, Marbun RA. Uji Senyawa Flavonoid Total Dari Ekstrak Etanol Herba Binara (Artemisia annua) Menggunakan High Performance Liquid Chromatography (HPLC) Tahun 2019. Jurnal Farmasimed (JFM). 2019 Oct 31;2(1):16-20.

[13] Departemen Kesehatan Republik Indonesia. (1995). Materi Pelatihan Teknologi Sediaan Herbal. Jakarta: Departemen Kesehatan RI.

[14] Syarifuddin AN, Zantrie R, Marbun RA. Identifikasi kadar vitamin C pada daging dan kulit buah naga merah (Hylocereus Polyrhizus) dengan metode spektrofotometri UV-Visible. Jurnal Farmasimed (Jfm). 2019 Oct 31;2(1):40-6.

[15] Syafitri, N., Abdurrahman Munir, M., Aprilia, V., & Emelda, E. (2023). Aplikasi Metode Titrasi Iodometri Untuk Determinasi Kadar Vitamin C Pada Jambu (Myrtaceae family). Fullerene Journ.Of Chem, 8(2), 38–43.

[16] Oktaria, D., & Marpaung, M. P. (2023). Penetapan kadar flavonoid total dan aktivitas antioksidan ekstrak akar nipah (nypa fruticans wurmb) dengan metode spektrofotometri uv-vis. Lantanida Journal, 11(1), 36.

[17] Indrawati, S., Lahming, L., & Sukainah, A. (2018). Analisis Sifat Fisiko Kimia Saus Cabai Fortifikasi Labu Siam Dan Labu Kuning. Jurnal Pendidikan Teknologi Pertanian, 4, 113.

[18] Hasanah, N., & Novian, D. R. (2020). Analisis Ekstrak Etanol Buah Labu Kuning (Cucurbita Moschata D.). Parapemikir : Jurnal Ilmiah Farmasi, 9(1), 54.

[19] Rahmaniati M, A., Ulfah, M., & Mulangsari, d. A. K. (2018). Standarisasi parameter non spesifik ekstrak etanol daun pegagan (centella asiatica l.) Di dua tempat tumbuh. Jurnal Inovasi Teknik Kimia, 3(1).

[20] Maryam, F., Taebe, B., & Toding, D. P. (2020). Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun Matoa (Pometia pinnata J.R & G.Forst). Jurnal Mandala Pharmacon Indonesia, 6(01), 1–12.

[21] Alzanando, R., Yusuf, M., & M.Si, T. (2022). Analisis Kadar Senyawa Alkaloid dan Flavonoid Total Ekstrak Etanol Daun Pepaya (Carica papaya L.) Menggunakan Spektrofotometri UV-Vis. Jurnal Farmasi Malahayati, 5(1), 108–120.

[22] Bachtiar, R. A., Handayani, S., & Roskiana Ahmad, A. (2023). Penetapan kadar flavonoid total buah dengen (dillenia serrata) menggunakan metode spektrofotometri uv-vis. Makassar Natural Product Journal, 1(2), 2023–2086.

[23] Helmidanora, R.-, Sukawaty, Y.-, & Warnida, H.-. (2020). Penetapan kadar flavonoid daun binahong (anredera cordifolia (ten) steenis) dengan metode spektrofotometri uv-vis. Scientia : Jurnal Farmasi Dan Kesehatan, 10(2), 192.

[24] Aminah, A., Tomayahu, N., & Abidin, Z. (2018). Penetapan kadar flavonoid total ekstrak etanol kulit buah alpukat (persea americana mill.) Dengan metode spektrofotometri uv-vis. Jurnal Fitofarmaka Indonesia, 4(2), 226–230.

[25] Riwanti, P., Izazih, F., & Amaliyah, A. (2018). Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50,70 dan 96% Sargassum polycystum dari Madura. Journal of Pharmaceutical-Care Anwar Medika, 2(2), 35–48.