Formulation and Evaluation of Feminine Hygiene Preparation from Lemongrass (Cymbopogon nardus) and Its Inhibitory Activity Against Candida albicans

Yosi Darmirani rani; Fitri Siska

doi:10.35451/a4bs3k68

Authors

Yosi Darmirani rani Institut Kesehatan Medistra Lubuk Pakam
Fitri Siska Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/a4bs3k68

Keywords:

Feminine hygiene, Flavonoids, Antifungal, Citronella (Cymbopogon nardus), Physical stability test

Abstract

Background: Fungal infections are a common health problem in tropical countries, including Indonesia, where the hot and humid climate favors the growth of pathogenic microorganisms. One of the fungi that frequently causes infections in women is Candida albicans, the main agent responsible for vaginal discharge. Synthetic antibacterial and antifungal compounds often cause side effects, adverse events, relatively high costs, and the risk of fungal resistance due to excessive use. This situation has encouraged people to return to natural ingredients (“back to nature”) as safer, more effective, and more economical alternatives. One plant with strong potential as a natural antifungal agent is citronella (Cymbopogon nardus). This plant is widely known as a cooking spice, massage oil ingredient, and traditional herbal remedy, and it is abundantly available in the Hutaginjang area, North Tapanuli Regency. Objective: This study aimed to formulate and evaluate a feminine hygiene preparation containing citronella extract and to test its antifungal activity against Candida albicans. Method: The research was conducted experimentally. Results: The results showed that all formulations met the physical property standards set by SNI, with viscosity ranging from 2000–50,000 cps and pH between 4.0–5.0, which is suitable for the vaginal area. Antifungal testing revealed that inhibition zones increased with higher extract concentrations—10%, 15%, and 20% extracts produced inhibition zones of 10.5 mm, 13.3 mm, and 17.1 mm, respectively. The 60% extract yielded a 22.5 mm inhibition zone (categorized as very strong), approaching that of the positive control ketoconazole (29.1 mm). Statistical analysis (Shapiro-Wilk, ANOVA, Tukey HSD, Duncan) indicated that the data were normal, homogeneous, and showed significant differences between treatments. Conclusion: It can be concluded that higher concentrations of citronella extract are more effective in inhibiting the growth of Candida albicans.

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References

1. Rezita N, Ambari Y, Nurrosyidah IH. Uji Efektivitas Antifungi Formulasi Sabun Cair Pembersih Kewanitaan (Feminine Hygiene) Ekstrak Etanol Daun Ceremai (Phyllanthus acidus (L.) Skeels) terhadap Pertumbuhan Candida albicans. J Islam Pharm. 2022;7(1):1–10.

2. Nadirah P, Destiara M, Istiqamah I. Etnobotani Serai Wangi (Cymbopogon nardus (L.) Rendle) Desa Batang Kulur Kecamatan Kelumpang Barat Kotabaru. Al Kawnu Sci Local Wisdom J. 2022;1(2):63–8.

3. Sari EK, Anantarini NPD, Dellima BREM. UJI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN SERAI WANGI (Cymbopogon nardus L.) SECARA IN VITRO DENGAN METODE HRBC (Human Red Blood Cell). Kartika J Ilm Farm. 2024;9(1):1–17.

4. Pratiwi PY. Formulasi DAN UJI stabilitas fisik sediaan sabun Cair pembersih kewanitaan (feminiNe hyegiene) ekstrak etanol herba pegagan (Centella asiatiCa (L) urb.). J Ilmu Kesehat Bhakti Setya Med. 2022;7(1):1–7.

5. Parwati D, Arif SD. Literasi Media Sosial dan Dampaknya pada Kesadaran Kebersihan Menstruasi di Kalangan Remaja Putri. J Sains, Farm dan Kesehat. 2024;01(01):27–51.

6. Solekha R, Purnobasuki H, Puspaningsih NNT, Setiyowati PAI. Secondary Metabolites and Antioxidants Activity from Citronella Grass Extract (Cymbopogon nardus L.). Indian J Pharm Educ Res. 2024;58(1):298–305.

7. Costa GM da, Santos MCC dos, Everton GO, Sousa PDCD de, Rangel MES. Physicochemical properties, toxicity and larvicidal activity of the essential oil of Cymbopogon winterianus in front of Aedes aegypti. Ciência e Nat. 2020;42:e39.

8. Widiawati I, Mulyati S. EFFLEURAGE MASSAGE MENGGUNAKAN ESENSIAL OIL SERAI (CYMBOPOGON Sp) DAN TEH SEREH EFEKTIF UNTUK MENURUNKAN NYERI PADA IBU POST PARTUM. J Ris Kesehat Poltekkes Depkes Bandung. 2021;13(1):230–8.

9. Yao H, Liu J, Jiang X, Chen F, Lu X, Zhang J. Analysis of the clinical effect of combined drug susceptibility to guide medication for carbapenem-resistant klebsiella pneumoniae patients based on the Kirby–Bauer disk diffusion method. Infect Drug Resist. 2021;14:79–87.

10. Lolok N, Awaliyah N, Astuti W. Formulasi Dan Uji Aktivitas Sediaan Sabun Cair Pembersih Kewanitaan Ekstrak Daun Waru (Hibiscus tiliaceus) Terhadap Jamur Candida albicans. J Mandala Pharmacon Indones. 2020;6(01):59–80.

11. Khamis WM, Heflish AA, El-Messeiry S, Behiry SI, Al-Askar AA, Su Y, et al. Swietenia mahagoni Leaves Extract: Antifungal, Insecticidal, and Phytochemical Analysis. Separations. 2023;10(5).

12. KURNIAWATI D. Uji Angka Kuman Dan Stabilitas Produk Sabun Cair Formulasi Herbal. Knowl J Inov Has Penelit dan Pengemb. 2022;2(2):72–8.

13. Marbun RAT. Asian Journal of Pharmaceutical Research and Development. Asian J Pharm Res Dev. 2020;8(6):77–80.

14. Tatiana WS, Kusumaningtyas A siwi, Ilham L. Literature Study of Three Plant Formulations with Anti-Candida Albicans Activity in Feminine Liquid. 2nd Int Conf Heal Sci Technol. 2021;(6 April):109–12.

15. Putri NI, Mardianingrum R, Susanti. FORMULASI DAN UJI AKTIVITAS ANTIJAMUR SABUN CAIR KEWANITAAN EKSTRAK ETANOL DAUN KETEPENG CINA (Cassia alata L.) TERHADAP Candida albicans. J Penelit Farm Indones. 2023;12(2):94–103.

16. Tanjung SA, Fatimah C, Khomariah PA. Formulasi Sediaan Vaginal Dauche Dengan Kombinasi Ekstrak Herba Bandotan ( Ageratum conyzoides L ) dan Bunga Kecombrang ( Etlingera elatior ). Parapemikir J Ilm Farm. 2025;14(1):95–106.

17. Westphal KR, Heidelbach S, Zeuner EJ, Riisgaard-Jensen M, Nielsen ME, Vestergaard SZ, et al. The effects of different potato dextrose agar media on secondary metabolite production in Fusarium. Int J Food Microbiol. 2021;347(March):109171.

18. Wisnianti D, Nasir N, Ode W. Uji Aktivitas Antijamur Fraksi N - Heksan , Etil Asetat dan Air Daun Rambutan Aceh ( Nephelium Lappaceum L .) Terhadap Pertumbuhan Candida Albicans Test of The Antifungal Activity Of N-Hexane , Ethyl Acetate and Water Fractions Aceh Rambuhan Leaves ( Neph. J Pharm Mandala Waluya. 2024;3(6):352–63.

19. Meyok GY, Jasman J. Uji Aktivitas Antijamur Minyak Kelapa Murni (Virgin Coconut Oil) Dan Minyak Kelapa Tradisional (Coconut Cooking Oil) pada Candida albicans. J Beta Kim. 2023;3(1):33–9.

20. Saputri NPD, Saputri GAR, Marcellia S. Formulasi Sediaan Salep Ekstrak Minyak Atsiri Daun Jeruk Purut (Citrus Hystrix DC.) terhadap Jamur Candida Albicans. JUMANTIK (Jurnal Ilm Penelit Kesehatan). 2022;6(4):337.

21. Purnamasari R, Marcellia S, Purnama RC. Uji Aktivitas Antifungi Ekstrak Etanol Daun Jambu Bol (Syzygium malaccense L.) dalam Sediaan Sabun Cair Kewanitaan Terhadap Candida albicans. J Pharm Trop Issues. 2021;1(4):96–101.

22. Octora DD, Marbun RA, Koto R. Uji aktivitas antibakteri ekstrak etanol daun pirdot (Saurauia vulcani Korth.) terhadap bakteri Salmonella thypi. Jurnal farmasimed (JFM). 2019 Nov 7;2(1):40-4.

23. Turnip NU, Anggraeni R, Sihombing YR, Wulandari S. Test to Determine The Quercetin Content of Tenggiang (Polystichum setiferum) Using the High Performance Liquid Chromatography (HPLC) Method and Its Antifungal Activity Against Pityrosporum ovale. JURNAL FARMASIMED (JFM). 2025 Apr 30;7(2):135-42.

24. Marbun RA. Uji aktivitas ekstrak daun pirdot (Sauraia vulcani Korth.) terhadap pertumbuhan Candida albicans secara in vitro. Jurnal Bios Logos. 2021;11(1):1-6.