Testing the Activity of Chitosan Nanohydrogel from Belut Sawah (Monopterus albus) Extract as a Future Anti-Aging Candidate
DOI:
https://doi.org/10.35451/x4nw7q34Keywords:
Nanokitosan, Physical Stability, Belut Sawah, Hydrogel, Anti-agingAbstract
Background: Premature aging has become a serious concern, especially among women. The aging process can occur at any time and progress rapidly. This condition may be caused by exposure to sunlight, which induces the formation of Reactive Oxygen Species (ROS) in the skin. When the amount of ROS exceeds the antioxidant defense capacity of skin cells, it can lead to dryness, thinning, loss of elasticity, wrinkles, and even skin cancer. The swamp eel (Monopterus albus) is commonly used as food by the community. Previous studies have shown that eel extract has been used to treat incision wounds, gastric ulcers, and anemia. It contains vitamin A, vitamin B, and selenium. This species is abundantly found in rice fields and muddy ponds in the Lubuk Pakam area. The formulation of the extract into nan chitosan derived from shrimp shells has been reported to improve the delivery of antioxidant effects into the skin layers. Objective: This study aimed to analyze the activity of a hydrogel combining swamp eel extract with nanochitosan, evaluate its antioxidant activity, and assess the physical stability of the hydrogel formulation as an anti-aging preparation. Methods: Extraction was carried out using the maceration method. The nanochitosan-based hydrogel formulation was designed to enhance the delivery of the eel’s active compounds. Antioxidant activity was tested using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method, and the physical stability of the hydrogel was evaluated. Results: The physical evaluation showed that the nanochitosan hydrogel containing swamp eel extract maintained good stability during storage. Organoleptic tests indicated a consistent yellowish-white color, characteristic aroma, and uniform texture (homogeneity) from day 0 to day 4. The pH value remained stable at 5.2 throughout weeks 0 to 4, within the physiological range of skin pH. Viscosity measurements were 10,350 cps for F1, 11,750 cps for F2, and 23,650 cps for F3. These findings suggest that the formulation possessed good physical stability and an appropriate consistency for topical application. Conclusion: Skin elasticity and biological activity tests showed an increase in skin firmness, likely due to the collagen, flavonoid, and antioxidant content in the swamp eel extract. Irritation and panelist preference tests confirmed its safety and user acceptability. Therefore, this hydrogel shows potential to be developed as a natural, safe, and consumer-friendly anti-aging formulation.
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[1]. Fitriani R, Lestari W, Putri A. Potensi protein dan kolagen dalam regenerasi jaringan luka bakar: Tinjauan literatur. Jurnal Farmasi dan Sains Terapan. 2021;8(2):101–10.
[2]. Handayani T, Sulastri D, Nuraini P. Formulasi nanogel ekstrak lidah buaya (Aloe vera) dan uji aktivitas penyembuhan luka bakar pada tikus putih. Jurnal Sains Farmasi & Klinis. 2022;9(1):45–54.
[3]. Haryanti R, Dewi K, Andini F. Efektivitas sediaan silver sulfadiazine dalam percepatan penyembuhan luka bakar. Jurnal Kedokteran dan Kesehatan. 2020;7(3):221–9.
[4]. Pratiwi D, Anggraini F, Widya R. Aktivitas salep madu lebah terhadap penyembuhan luka bakar pada tikus putih (Rattus norvegicus). Jurnal Penelitian Kesehatan. 2020;12(2):67–75.
[5]. Sari D, Lubis H, Fauziah R. Gel ekstrak ikan gabus (Channa striata) sebagai terapi luka bakar: Studi in vivo pada tikus putih. Jurnal Biomedis dan Farmasi. 2021;5(1):33–42.
[6]. Wullur VM, Datu VV, Kristin Y, Kolang F, Takahindangen CJ, Komaling AV, et al. Efektivitas nanogel ekstrak kulit pisang Goroho (Musa acuminata L.) terhadap luka bakar pada tikus putih (Rattus norvegicus). Jurnal Biomedik dan Farmasi. 2025;13(2):95–100.
[7]. Tsai SY, Lio CF, Yao WC, Liu CP, Shih SC, Wang TYT, et al. Cost-drivers of medical expenses in burn care management. Burns [Internet]. 2020;46(4):817–24. Available from: https://doi.org/10.1016/j.burns.2020.01.004
[8]. Markiewicz-Gospodarek A, Kozioł M, Tobiasz M, Baj J, Radzikowska-Büchner E, Przekora A. Burn wound healing: Clinical complications, medical care, treatment, and dressing types: The current state of knowledge for clinical practice. Int J Environ Res Public Health. 2022;19(3):1–15.
[9]. Pradita EY, Wahyuni S. Synthesis of chitosan-alginate-Siam orange (Citrus nobilis Lour) extract and its antibacterial activity. Indones J Chem Sci. 2023;12(1):58–69.
[10]. Dewi NPDC, Rata KPP, Gresitha MS, Pradnaya IG. Combination of PVA chitosan collagen membrane and Moringa oleifera nanogel for oral wound healing: A literature review. Bali Dent Sci Exhib. 2024;1(1):1–9.
[11]. Ali A, Ali A, Rahman MA, Warsi MH, Yusuf M, Alam P. Development of nanogel loaded with lidocaine for wound-healing: Illustration of improved drug deposition and skin safety analysis. Gels. 2022;8(8):1–12.
[12]. Kamenova K, Radeva L, Yoncheva K, Ublekov F, Ravutsov MA, Marinova MK, et al. Functional nanogel from natural substances for delivery of doxorubicin. Polymers (Basel). 2022;14(17):1–13.
[13]. Elkomy MH, Alruwaili NK, Elmowafy M, Shalaby K, Zafar A, Ahmad N, et al. Surface-modified bilosomes nanogel bearing a natural plant alkaloid for safe management of rheumatoid arthritis inflammation. Pharmaceutics. 2022;14(3):1–14.
[14]. Nurlita L, Sari WY, Ramadhan F. Studi etnobotani tumbuhan berkhasiat obat. J Farmasetis. 2023;12(4):457–72.
[15]. Alam MA, Khan MA, Sarower-e-Mahfuj M, Ara Y, Parvez I, Amin MN. A model for tubificid worm (Tubifex tubifex) production and its effect on growth of three selected ornamental fish. Bangladesh J Fish. 2022;33(2):205–14.
[16]. Agustiani FRT, Sjahid LR, Nursal FK. Kajian literatur: Peranan berbagai jenis polimer sebagai gelling agent terhadap sifat fisik sediaan gel. Maj Farmasetika. 2022;7(4):270–8.
[17]. Yanti S, Arif MS, Yusuf B. Sintesis dan stabilitas nanopartikel perak (AgNPs) menggunakan trinatrium sitrat. Pros Semin Nas Kim. 2021;2:1–5.
[18]. Rosilina M, Maulana MT, Astuti DH. Sintesis dan modifikasi ukuran partikel nano-PCC dengan penambahan etilen glikol. Chempro. 2023;3(1):45–50.
[19]. Chan WS, Santobuono M, D’Amico E, Selck H. The antidepressant sertraline impacts growth and reproduction in the benthic deposit feeder Tubifex tubifex. Ecotoxicol Environ Saf [Internet]. 2024;285:117134. Available from: https://doi.org/10.1016/j.ecoenv.2024.117134
[20]. Saputra D. Tinjauan komprehensif tentang luka bakar dan penanganannya. J Sci Univ Andalas Padang [Internet]. 2023;12:207–18. Available from: http://journal.scientic.id/index.php/sciena/issue/view/12
[21]. Beda SJ, Ndaong NA, Almet J. Uji aktivitas ekstrak daun akasia (Acacia auriculiformis) sebagai antihelmintik terhadap cacing Ascaris suum. J Vet Nusant. 2022;5(2):11–21. Beda SJ, Ndaong NA, Almet J. Uji aktivitas ekstrak daun akasia (Acacia auriculiformis) sebagai antihelmintik terhadap cacing Ascaris suum. J Vet Nusant. 2022;5(2):11–21.
[22]. Nnamani PO, Ugwu AA, Nnadi OH, Kenechukwu FC, Ofokansi KC, Attama AA, et al. Formulation and evaluation of transdermal nanogel for delivery of artemether. Drug Deliv Transl Res [Internet]. 2021;11(4):1655–74. Available from: https://doi.org/10.1007/s13346-021-00951-4
[23]. Nurjanah L, Handayani T, Fitriani D. Pengaruh kitosan terhadap sifat adhesivitas sediaan gel herbal. Jurnal Farmasi Klinik Indonesia. 2020;14(2):77–85.
[24]. Wulandari S, Handayani T, Fitriani D. Efek gel protein ikan gabus terhadap elastisitas kulit tikus. Jurnal Biomedik dan Farmasi. 2021;22(1):33–40.
[25]. Handayani T, Fitriani D, Nurjanah L. Formulasi dan evaluasi sediaan gel topikal untuk anti-aging. Jurnal Ilmu Farmasi. 2021;19(4):201–10.
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