Anti-Inflammatory Activity of Ethanol Extract of Turmeric Rhizome (Curcuma Longa Linn) Male White Mice Pepper Induced Caragenan

Nur Ulina M. Br. Turnip; Sefrini Ois Lasmaria Pasaribu

doi:10.35451/3n8wb204

Authors

Nur Ulina M. Br. Turnip Institut Kesehatan Medistra Lubuk Pakam
Sefrini Ois Lasmaria Pasaribu Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/3n8wb204

Keywords:

Turmeric Extract, Anti-inflammatory, Carrageenan, Inflammation Inhibition

Abstract

Background: Inflammation is the body's natural response to tissue injury caused by internal or external stimuli, with the aim of neutralizing the agent causing damage and repairing injured tissue. Initial treatment of inflammation is generally done by eliminating local triggering factors, such as pain relievers through the use of chemical drugs. However, this therapy often causes unwanted side effects. The use of natural ingredients that have anti-inflammatory activity is a safer alternative than modern drugs. Objective: to determine the anti-inflammatory effect of ethanol extract of turmeric rhizome (Curcuma longa L.). Method: using a model of edema on the soles of the feet of male white mice induced by 1% carrageenan. A total of 24 mice were divided into 6 treatment groups, one group was not given treatment as a comparison, three groups received doses of ethanol extract of turmeric rhizome (100, 200, and 300 mg/kgBW), one negative control (Na CMC 0.5%), and one positive control (6.5 mg/kgBW Na diclofenac). Results: showed that after carrageenan induction, all test groups experienced a progressive decrease in inflammation levels from the 60th to the 360th minute. After the extract dose groups of 100 mg/kgBW, 200 mg/kgBW, 300 mg/kgBW, and Na diclofenac, the Na CMC group had the highest percentage of inflammation at the 120th minute. The Na diclofenac group showed the highest percentage of inflammation inhibition, followed by extract doses of 300, 200, and 100 mg/kgBW. In contrast to the Na CMC group which did not show anti-inflammatory activity, these data indicate that the ethanol extract of turmeric rhizome at a dose of 300 mg/kgBW has the highest anti-inflammatory efficacy after Na diclofenac, followed by doses of 200 mg/kgBW and 100 mg/kgBW. Conclusion: turmeric rhizome extract has the potential to reduce inflammation in the soles of mice's feet.

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References

[1] Yunita F. The journey of Indonesian traditional medicine. Tarumanagara Med J. 2024;6(2):241-252

[2] Setiawan A. Keanekaragaman hayati Indonesia: Masalah dan Upaya Konservasinya. Indonesian Journal of Conservation. 2022. 13-21.

[3] Hildasari F, Hayati N. Potensi keanekaragaman flora sebagai tumbuhan obat di Wana Wiyata Widya Karya, Sanggar Indonesia Hijau, Kabupaten Pasuruan. Jurnal Biologi dan Pembelajarannya (JBioP). 2021;8(2):97–104.

[4] Halimatushadyah E, Rahayu A. Anticancer activity of turmeric rhizome extract (Curcuma longa Linn) in vitro against MCF7 breast cancer line cells. Health Information: Jurnal Penelitian. 2023;15(2):164-171.

[5] Chen S, Wang X, Cheng Y, Gao H, Chen X. A review of classification, biosynthesis, biological activities and potential applications of flavonoids. Molecules. 2023;28(13):4982.

[6] Al-Khayri JM. Flavonoids as potential anti-inflammatory molecules. Molecules. 2022;27(9):2901.

[7] Wasiaturrahmah Y, Amalia N. Potensi antiinflamasi ekstrak daun kecapi sentul (Sandoricum koetjape Merr) dengan metode stabilisasi membran sel darah merah. JIIS (Jurnal Ilmiah Ibnu Sina): Ilmu Farmasi dan Kesehatan. 2022;8(1):1277.

[8] Fahmi FNA, Putra B, Santi I. Uji efek antiinflamasi ekstrak etanol daun matoa (Pometia pinnata J.R Forst & G. Forst) pada tikus jantan (Rattus norvegicus). Makassar Natural Product J. 2023;1(2(6)):33–43.

[9] Mus NM. Anti-inflammatory activity of dysmenorrhea herbal preparation: mechanisms and mediator involvement. Indonesian Journal of Pharmaceutical Science and Technology. 2024;11(2):87–96.

[10] Khalil NA, Ahmed EM, Tharwat T, Mahmoud Z. NSAIDs between past and present; a long journey towards an ideal COX-2 inhibitor lead. RSC Adv. 2024;14:30647-30661.

[11] Sari DK, Puspitasari IM, Anggraini DI. Mekanisme kerja obat antiinflamasi nonsteroid (NSAID) dalam menghambat proses inflamasi. Jurnal Ilmu Kefarmasian Indonesia. 2021;19(2):145–152.

[12] Kinanti R. Efektivitas obat herbal sebagai alternatif terapi antiinflamasi. Yogyakarta: Universitas Gadjah Mada Press; 2016.

[13] Apriliyani MW, Suryanto D, Kurniawan R. Aktivitas antibakteri ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Streptococcus mutans. Jurnal Ilmiah Farmasi. 2023;19(2):115–123.

[14] Rahmawati N, Lestari D, Hidayat R. Optimasi proses maserasi ekstrak etanol daun salam (Syzygium polyanthum (Wight) Walp.) terhadap rendemen dan aktivitas antioksidan. Jurnal Farmasi Indonesia. 2022;19(3):210–218.

[15] Megawati D, Sahea H R, Laya A. R. Skrining fitokimia dan uji antioksidan fraksi etil asetat dari ekstrak etanol daun kunyit (Curcuma longa L.) dengan metode DPPH. An-Najat. 2023;3(3)

[16] Pradita T, Fitriani Y, Rahmawati N. Uji daya antiinflamasi ekstrak etanol daun Mangkokan (Nothopanax scutellarium Merr.) pada mencit yang diinduksi karagenin. Jurnal Ilmiah Farmasi Farmasyifa. 2022;5(2):101–108.

[17] Royani S, Yuliyanti S. Identifikasi kualitatif senyawa metabolit sekunder pada rimpang kunyit (Curcuma longa L.) di Kabupaten Banyumas melalui skrining fitokimia. Jurnal Bina Cipta Husada: Jurnal Kesehatan dan Sains. 2022

[18] Purba N, Harianja BA, Akbar K, Harefa K. Uji aktivitas antiinflamasi ekstrak etanol daun jeruk nipis (Citrus aurantiifolia) pada mencit jantan (Mus musculus) yang diinduksi karagenan. J Farm Medistra (JFM). 2022;5(1):14–21.

[19] Lestari D, Wahyuni T, Widyaningsih W. Tinjauan mekanisme kurkuminoid dalam penghambatan inflamasi melalui jalur COX dan LOX. Jurnal Ilmu Kefarmasian Indonesia. 2020;18(1):45–53.

[20] Rahmawati N, Prasetyo BF, Nurjanah S. Aktivitas antiinflamasi dan antioksidan senyawa kurkumin pada rimpang Curcuma longa L. Jurnal Fitofarmaka Indonesia. 2021;8(2):112–120.