Comparative Study of Antioxidant Test of Combination Pomegranate Peel and Rice Husk Extract in Body Scrub Formula

Nadya Tsurayya; Risca Wulandari

doi:10.35451/467hrw03

Authors

Nadya Tsurayya Gunadarma University
Risca Wulandari Gunadarma University

DOI:

https://doi.org/10.35451/467hrw03

Keywords:

Pomegranate peel, Rice husk, Antioxidants, Body scrub, Combined extracts

Abstract

Background: Antioxidant molecules have the ability to convert free radicals into harmless molecules. Antioxidants can be formed in the body or obtained from external sources, either in the form of enzymes, minerals, or non-enzymatic compounds such as vitamins A, C, and E. Pomegranate peel contains bioactive compounds in the form of phenolics, flavonoids, and tannins. The use of rice husks is relevant because, as a natural exfoliator, they also contain phenolic/flavonoid antioxidants. Research objective: To assess and compare the antioxidant activity of body scrubs made from rice husk extract and pomegranate peel. Research method: The methods used were maceration, antioxidant testing using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and physical stability testing of the preparations. The antioxidant activity test results showed that the IC50 value of pomegranate peel extract was 7.65 ppm. Results: The antioxidant activity test results of preparations combining the extract with rice husks showed an IC50 value of 3.65 ppm. This indicates that F3 has very strong antioxidant activity. Based on organoleptic testing, homogeneity, pH, spreadability, adhesiveness, and cream type in Formulas 0, 1, 2, and 3. Conclusion: Pomegranate peel extract and rice husks can be successfully combined to produce a stable body scrub formula with sustained antioxidant activity

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References

[1] Innaya AY, Rohmawati NV, Ramadhani MW, Raisya N, Hidayah U, Faisal. Uji Skrining Fitokimia pada Kulit Delima Merah (Punica Granatum L.)di Taman Alquran Universitas Islam Malang. Era Sains J Sci Eng Inf Syst Res. 2024;2(1):30–8.

[2] Fadlilah AR, Lestari K. Review : Peran Antioksidan Dalam Imunitas Tubuh. Farmaka. 2023;21(2):171–8.

[3] Sharifi-Rad J, Quispe C, Castillo CMS, Caroca R, Lazo-Vélez MA, Antonyak H, et al. Ellagic Acid: A Review on Its Natural Sources, Chemical Stability, and Therapeutic Potential. Oxid Med Cell Longev. 2022;2022.

[4] Tumbarski Y, Ivanov I, Vrancheva R, Mazova N. Pomegranate Peels : A Promising Source of Biologically Active Compounds with Potential Application in Cosmetic Products. 2025;1:1–19.

[5] Wisetkomolmat J, Arjin C, Hongsibsong S, Ruksiriwanich W, Niwat C, Tiyayon P, et al. Antioxidant Activities and Characterization of Polyphenols from Selected Northern Thai Rice Husks: Relation with Seed Attributes. Rice Sci. 2023;30(2):148–59.

[6] Dira MA, Dewi KMC. Formulasi dan Evaluasi Krim Body Scrub Kombinasi Ekstrak Moringa oleifera dan Oryza sativa Sebagai Eksfolian. J Mandala Pharmacon Indones. 2022;8(2):307–17.

[7] Iskandar B, Tarigan J, Leny L, Hanum W. Uji Sifat Fisik Sediaan Lulur Ekstrak Bayam Merah (Amaranthus tricolor L.) Serta Uji Efektivitas Kelembaban (Moisture) Dan Kehalusan (Evenness) Pada Kulit. Maj Farmasetika. 2023;9(1):104.

[8] Hairiyah N, Amalia RR, Nuryati N. Karakterisasi dan penentuan formulasi terbaik bodyscrub berbasis ampas kelapa dengan penambahan esktrak bunga telang (Clitoria ternatea). Agrointek J Teknol Ind Pertan. 2025;19(2):394–401.

[10] Gulcin İ, Alwasel SH. DPPH Radical Scavenging Assay. Processes. 2023;11(8).

[11] Munteanu IG, Apetrei C. Analytical methods used in determining antioxidant activity: A review. Int J Mol Sci. 2021;22(7).

[12] Luthfianto D, Noviyanti RD, Hastuti W, Pertiwi D, Kusudaryati D. PROFESI ( Profesional Islam ): Media Publikasi Penelitian ; 2024 ; Volume 22 ; No 2 Website : https://journals.itspku.ac.id/index.php/profesi/ Ekstraksi Maserasi Bligo ( Benincasa hispida ) Menggunakan Pelarut Etanol Bligo Maceration Extraction Using Etha. 2024;22(2):78–84.

[13] Budiarti ZT, Agustin EW. Kelayakan Limbah Sekam Padi (Orysa Sativa L.) sebagai Bahan Tambahan Sediaan Sabun Mandi Padat Alami. Beauty Beauty Heal Educ. 2023;12(1):21–32.

[14] Supriningrum R, Fatimah N, Purwanti YE. KARAKTERISASI SPESIFIK DAN NON SPESIFIK EKSTRAK ETANOL DAUN PUTAT (Planchonia valida). Al Ulum J Sains Dan Teknol. 2019;5(1):6.

[15] Ningtyas RH, Erwiyani AR. Formulasi dan Uji Stabilitas Fisik Sediaan Permen Jeli Ekstrak Wortel (Daucuscarota L.). Indones J Pharm Nat Prod. 2023;6(01):15–23.

[16] Wardhani RRAAK, Akhyar O, Prasiska E. Screening of Phytochemical, Antioxidant Activity and Total Phenolic-Flavonoid of Leaves and Fruit Extract of Galam Rawa Gambut (Melaleuca cajuputi ROXB). QUANTUM J Inov Pendidik Sains. 2018;9(2):133–43.

[17] Bachtiar RA, Handayani S, Roskiana Ahmad A. PENETAPAN KADAR FLAVONOID TOTAL BUAH DENGEN (Dillenia serrata) MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS. Makassar Nat Prod J [Internet]. 2023;1(2):2023–86. Available from: https://journal.farmasi.umi.ac.id/index.php/mnpj

[18] Laila Rahma L, Suryanti L. Formulasi Dan Uji Stabilitas Fisik Sediaan Body Scrub Bengkuang (Pachyrhizus Erosus) Dengan Variasi Konsentrasi Emulgator. J Ilm Farm Indones [Internet]. 2023;1(1):12–20. Available from: www.uima.ac.id

[19] Latifah SL, Pudjono P, Rosmi RF. Formulasi dan Evaluasi Mutu Fisik Sediaan Body Scrub Cream Varietas Ubi Jalar dalam Fase Air dan Minyak. Pharm Perad J. 2022;2(1):20–32.

[20] Suciati A, Marfu N, Sawitri SB. FORMULATION AND EVALUATION OF LIP BALM PREPARATION COMBINATION OF AVOCADO OIL AND RED BEETROOT EXTRACT ( Beta vulgaris L .) FORMULASI DAN EVALUASI SEDIAAN LIP BALM KOMBINASI MINYAK ALPUKAT DAN EKSTRAK UMBI BIT MERAH ( Beta vulgaris L .). 2025;8(2):356–68.

[21] Mutmainna Tamrin, Achmad Kadri Ansyori, Hayatus Sa’adah. Uji Aktivitas Antioksidan Ekstrak Etanol Biji Buah Nyirih (Xylocarpus granatum) dengan Metode DPPH Secara Spektrofotometri UV-VIS. J Ris Kefarmasian Indones. 2024;6(2):233–48.

[22] Kadar P, Ekstrak A, Senggani D. p ISSN : 2775-8567 p ISSN : 2775-8567. 2024;04(1):49–55.

[23] Hari DG, Lovena TN, Khairiah U. Penetapan Kadar Flavonoid Ekstrak Etanol Daun Labu Kuning ( Cucurbita moschata Dulchesne ) Menggunakan Spektrofotometri Uv-Vis Determination of Flavonoid Content of Ethanol Extract of Yellow Pumpkin Leaves ( Cucurbita moschata Dulchesne ) Using Uv-Vis Spectrophotometry. :191–7.

[24] Silverman M, Lee PR, Lydecker M. Formularies. Pills and the Public Purse. 2023;97–103.

[25] Qomaliyah EN, Indriani N, Rohma A, Islamiyati R. Skrining Fitokimia, Kadar Total Flavonoid dan Antioksidan Daun Cocor Bebek. Curr Biochem. 2023;10(1):1–10.

[26] Derakhshan Z, Ferrante M, Tadi M, Ansari F, Heydari A, Hosseini MS, et al. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food Chem Toxicol [Internet]. 2018;114:108–11. Available from: https://doi.org/10.1016/j.fct.2018.02.023

[27] Panapitiya PDSNH, Weerasooriya MKB, Punchipatabendhi TI. Antioxidant Capacity and Nutritional Value of Peels and Seeds of Selected Pomegranate (Punica granatum L.) Cultivars from Sri Lanka. J Agric Sci - Sri Lanka. 2022;17(3):379–87.

[28] Nurmazaya V, Nurlaila R, ZA N, Hakim L, Dewi R, Fibarzi WU. Analisis Pengaruh Suhu Dan Waktu Pada Pembentukkan Silika Dari Sekam Padi Menggunakan Larutan Koh. Chem Eng J Storage. 2023;2(5):68–78.

[29] Sianipar MA, Harahap HY, Aritonang B. Formulasi Sediaan Body Scrub yang Mengandung Ekstrak Etanol Rimpang Temulawak ( Curcuma xanthorrhiza Roxb ) sebagai Pelembab Kulit Formulation of Body Scrub Containing Ethanol Extract of Temulawak Rhizome ( Curcuma xanthorrhiza Roxb ) as a Skin Moisturizer. 2025;(c).

[30] Firmansyah F, Adriana ANI, Narni N. FORMULASI DAN UJI MUTU FISIK SEDIAAN KRIM BODY SCRUB EKSTRAK KULIT PISANG GOROHO (Musa acuminate L.). Pharmacol Pharm Sci Journals. 2023;2(1):30–8.

[31] Akmal T, Pratama BA. Formulasi Dan Evaluasi Sediaan Krim Anti-Aging Ekstrak Kulit Buah Mangga Harum Manis (Mangifera indica L.). Pharm Sci Clin Pharm. 2023;1(2):37–43.

[32] Nurhidayati LG, Rejeki DS, Novi S, Fauziah N. Formulasi dan Evaluasi Sediaan Body Scrub Bunga Telang ( Clitoria ternatea L .) dan Sabut Kelapa ( Cocos nucifera L .) sebagai Antioksidan. 2024;10(2):697–706.

[33] Hidayati W. FORMULASI DAN EVALUASI UJI MUTU FISIK BODY SCRUB DARI EKSTRAK BUNGA KECOMBRANG (Etlingera elatior). JKPharm J Kesehat Farm. 2023;5(1):40–55.

[34] Situmorang NBR, Anna R, Marbun T. Formulasi dan Evaluasi Nanocream Ekstrak Maggot ( Hermatia illucens ) Sebagai Kandidat Anti-Aging Masa Depan Formulation and Evaluation of Maggot Extract Nanocream ( Hermatia illucens ) as a Future Anti-Aging Candidate. (c).