The Immunomodulatory Potential of Ethanolic Extract of Blue Butterfly Pea Flower (Clitoria ternatea) on Non-Specific Immune Mechanisms

Ermalyanti Fiskia; Muhammad Azhar A  Karim; Muhammad Fakhrur Rajih  Hi Yusuf; Rufaidah Azzahra; Ismail Rahman

doi:10.35451/s67fbc03

Authors

Ermalyanti Fiskia Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Khairun
Muhammad Azhar A Karim Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Khairun
Muhammad Fakhrur Rajih Hi Yusuf Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Khairun
Rufaidah Azzahra Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Khairun
Ismail Rahman Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Khairun

DOI:

https://doi.org/10.35451/s67fbc03

Keywords:

Blue Butterfly Pea, Immunomodulator, carbon clearance, Lymphoid organ

Abstract

The immune system is a complex mechanism consisting of cells, tissues, and organs that work in coordination to protect the body from exposure to pathogens. However, pathogens also possess strategies to counteract immune defenses, thereby necessitating external substances to support immune enhancement. One such substance is antioxidants. Butterfly pea flower (Clitoria ternatea) is a potential candidate as an immunomodulator due to its high antioxidant content, which functions to prevent lipid peroxidation and combat free radicals. This study aims to investigate the immunomodulatory potential of the ethanol extract of butterfly pea flower using the carbon clearance test in mice. The research method was experimental, employing 30 BALB/c mice as test animals, which were divided into six groups: Butterfly Pea Flower Extract (EBTB) at doses of 150, 300, and 600 mg/kg BW, levamisole 25 mg and methylprednisolone 4 mg as comparison groups, and sodium CMC as the negative control group. The substances were administered orally for seven consecutive days, followed by intravenous induction of carbon ink on day 8. The obtained serum was measured for absorbance using UV-Vis spectrophotometry at 650 nm. Parameters observed included the phagocytic index (PI) and lymphoid organ index (liver, spleen, and thymus). Based on the phagocytic index calculation, EBTB at doses of 150 and 300 mg/kg BW yielded values of 2.990 and 2.929, respectively, indicating strong immunostimulant activity, while BPFE at 600 mg/kg BW showed moderate immunostimulant activity with a PI value of 1.423. Results for the lymphoid organ index in the BPFE 150 group did not show significant differences (P > 0.05) compared to the control group. In conclusion, the ethanol extract of butterfly pea flower demonstrates potential as an immunomodulator for nonspecific immune responses.

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References

[1] Grayson MH. Basic immunology Literature Review. Annals of Allergy, Asthma & Immunology 2023;130:125–6. https://doi.org/10.1016/j.anai.2022.09.018.

[2] Pannu N, Bhatnagar A. Oxidative stress and immune complexes: Pathogenic mechanisms in pristane induced murine model of lupus. Immunobiology 2020;225:151871. https://doi.org/10.1016/j.imbio.2019.11.006.

[3] Asfianti V, Sapitri A, Marbun ED. The immunomodulatory activity of ethanol extract of attarasa bark and fruit (Litsea cubeba (lour.) pers.) toward carbon clearance of mice (Mus musculus). Scientific Journal of Pharmacy 2022;special edition:175–84.

[4] Luhurningtyas FP, Dyahariesti N, Fitri Eka S. Uji Efek Imunomodulator Ekstrak Biji Karika (Carica pubescens Lenne K. Koch) terhadap Peningkatan Aktivitas Fagositosis pada Mencit Putih Swiss Webster. Pharmaceutical and Biomedical Sciences Journal 2020;2:27–34.

[5] Marshall JS, Warrington R, Watson W, Kim HL. An introduction to immunology and immunopathology. Allergy Asthma Clin Immunol 2018;14:49. https://doi.org/10.1186/s13223-018-0278-1.

[6] Guli MM. Respon Imun Hospes Terhadap Infeksi Vibrio cholerae. Biocelebes 2021;15. https://doi.org/110.22487/bioceb.v15i2.15777.

[7] Puspitaningrum I, Dwi Franyoto Y, Munisih S. Aktivitas Imunomodulator Fraksi Etil Asetat Daun Som Jawa (Talinum paniculatum (Jacq.) Gaertn) Terhadap Respon Imun Spesifik. Jurnal Ilmu Farmasi Dan Farmasi Klinik 2018;15:48–53.

[8] Hidayah IN, Indradi RB. Review Artikel: Aktivitas Imunomodulator Beberapa Tanaman Dari Suku Zingiberaceae. Jurnal Kesehatan Bakti Tunas Husada : Jurnal Ilmu Ilmu Keperawatan, Analis Kesehatan Dan Farmasi 2020;20. https://doi.org/https://doi.org/10.36465/jkbth.v20i2.610.

[9] Putra B, Azizah RN, Nopriyanti EM. Effect of Immunomodulator of Purslane Herb Ethanol (Portulaca oleracea L.) on Rat (Rattus norvegicus) With Delayed Type Hypersensitivity (DTH) Parameter. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 2020;6:20–5. https://doi.org/10.22487/j24428744.2020.v6.i1.14106.

[10] Pratama NH, Fadhilah A. Immunomodulatory Activity Test of Methanol Extract of Combrang Fruit (Etlingera elatior (Jack) R.M.Smith) Against Non-Specific Immune Response in Mice By Carbon Clearance Method. Usadha: Journal of Pharmacy 2022;1.

[11] Fadhilah A, Dwi Parwati A, Hanif Muflihah C, Sri Wahyuni A. Immunomodulatory Activity of Kasumba Turate (Carthamus tinctorius L.) Extract Against Non-Specific and Specific Immune Responses in Mice. Pharmacon: Jurnal Farmasi Indonesia 2023;20:61–7. https://doi.org/10.23917/pharmacon.v20i1.22629.

[12] Suwarna HK, Zainah NY, Putri RG, Umami M. Uji Fitokimia Ekstrak Daun Telang (Clitoria ternate L.) Menggunakan Metode Tabung. Jurnal Teknologi Pangan dan Ilmu Pertanian 2024;2:91–8. https://doi.org/10.59581/jtpip-widyakarya.v2i2.3705.

[13] Rahmatillah A, Fitriawati A, Putri AFK. Synergistic Impact of The Combination of Moringa Leaves and Butterfly Pea Flowers Infusion on Cognitive Enhancement. Jurnal Farmasimed (JFM) 2025;8:320–30. https://doi.org/10.35451/n9214f64.

[14] Harahap S, Siska Futri Nasution. Innovation of Banana Blossom Herbal Tea in Enhancing Interleukin-1 and Interleukin-2 Levels as an Immunomodulatory Approach for Cancer Prevention. Jurnal Farmasimed (JFM) 2025;8:236–42. https://doi.org/10.35451/cts2c934.

[15] Vifta RL, Winarti N, Rahayu S. Flavonoid Total Dan Potensi Antioksidan Bunga Telang (Clitoria Ternatea L.) Sebagai Tanaman Fungsional Kabupaten Semarang. Media Informasi Penelitian Kabupaten Semarang (SINOV) 2020;3. https://doi.org/https://doi.org/10.55606/sinov.v3i1.72.

[16] Harahap S, Siska Futri Nasution. Innovation of Banana Blossom Herbal Tea in Enhancing Interleukin-1 and Interleukin-2 Levels as an Immunomodulatory Approach for Cancer Prevention. Jurnal Farmasimed (JFM) 2025;8:236–42. https://doi.org/10.35451/cts2c934.

[17] Roseno M, Sudaryat Y, Widyastiwi. Immunomodulatory Activity of Ethanolic Extract of Kemukus (Piper cubeba), Kiseureuh (Piper aduncum), dan Cabe Jawa (Piper retrofractum) in Balb/C Mice. Jurnal Ilmu Kefarmasian Indonesia 2019;17. https://doi.org/https://doi.org/10.35814/jifi.v17i2.558.

[18] Utama RF, Rosidah, Yuandani. Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method. Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR) 2020;03:19–24.

[19] Utama RF, Rosidah, Yuandani. Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method. Indonesian Journal of Pharmaceutical and Clinical Research 2020;3:19–24. https://doi.org/10.32734/idjpcr.v3i2.4306.

[20] Nur A, Sadik F, Abdullah A, Rajih MF, Fiskia E, Disi MZA, et al. Telang Biru (Clitoria ternatea) Potensi Herbal Dalam Farmasi Modern. 1st ed. Purwokerto: Pena Persada; 2024.

[21] Asworo RY, Widwiastuti H. Pengaruh Ukuran Serbuk Simplisia dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Sirsak. Indonesian Journal of Pharmaceutical Education 2023;3. https://doi.org/10.37311/ijpe.v3i2.19906.

[22] Faradilla M, Fidrianny I, Iwo MI. Antioxidant and immunomodulatory activities of ethanol extracts from Syzygium cumini L. Skeels and Pogostemon cablin Benth n.d. https://doi.org/10.52225/narra.v4i3.e918.

[23] Sujono TA, Kusumowati, DIT, Munawaroh R. Immunomodulatory Activity of Muntingia calabura L. Fruits Using Carbon Clearance Assay and Their Total Flavonoid and Phenolic Contents. Asian Journal of Pharmaceutical and Clinical Research 2019:140–5. https://doi.org/10.22159/ajpcr.2020.v13i2.36449.

[24] Grennan D, Wang S. American Medical Association: Steroid Side Effects. Am Fam Physician, vol. 58, JAMA; 2019, p. 443–50.

[25] Srivastava R, Mishra MK, Patel AK, Singh A, Kushwaha K. An insight of non-steroidal anti-inflammatory drug mefenamic acid: A review. GSC Biological and Pharmaceutical Sciences 2019;7:052–9. https://doi.org/10.30574/gscbps.2019.7.2.0066.

[26] Fokunang C. Overview of non-steroidal anti-inflammatory drugs (nsaids) in resource limited countries. MOJ Toxicol 2018;4:5–13. https://doi.org/10.15406/mojt.2018.04.00081.

[27] Putra ON, Anggraini MA, Hardiyono H. Efek Obat Imunosupresan Pada Pasien Autoimun dengan COVID-19 (A Scoping Review of The Clinical Evidence). Journal of Islamic Pharmacy 2021;6:88–94. https://doi.org/10.18860/jip.v6i2.12884.

[28] Sujono TA, Fakhrudin DM. Imunomodulator Activity Of Ethanol-70% Extract Of Talok Leaves (Muntingia calabura L.) On Non-Specific Immune Response In Male Swiss Webster Mice. Usadha: Journal of Pharmacy 2024;3:104–14. https://doi.org/https://doi.org/10.23917/ujp.v3i1.320.

[29] Alipin K, Rina Nur Azizah N. Morfologis dan Beratrelatif Organ Hati Tikus Yang Diinduksi Karagenan Setelah Pemberian Ekstrak Kombinasi Rimpang Temulawak Dan Buah Belimbing Wuluh. Seminar Nasional Pendidikan Biologi dan Saintek, Bandung: 2021, p. 243–7.

[30] Maisaroh S, Harjana T. Pengaruh Kombinasi Ekstrak Daun Bayam Merah (Amaranthus tricolor, L.) Dan Biji Kacang Polong (Pisum sativum, L.) Terhadap Struktur Histologi Hati, Ginjal dan Lambung Tikus Putih Betina (Rattus norvegicus). The Journal of Biological Studies 2023;9:92–108.

[31] Dewa I, Angga G, Nata T, Linawati NM, Ayu G, Ratnayanti D, et al. Efek Pemberian Teh Kombinasi Bunga Euphorbia Milii dan Propolis Terhadap Diameter Pulpa Putih Limpa Tikus Wistar Jantan. Jurnal Medika Udayana 2021;10:1–7. https://doi.org/10.24843.MU.2020.V10.i5.P01.

[32] Tarantino G, Citro V. Crosstalk Between the Spleen and Other Organs/Systems: Downstream Signaling Events. Immuno 2024;4:479–501. https://doi.org/10.3390/immuno4040030.

[33] Sihombing YR, Fitri Siska. Activity Test of Daun Jati Belanda (Guazuma ulmifolia Lamk.) on Lymphocyte Proliferation and Immunoglobulin G Expressionas an Immunostimulatory Agent. Jurnal Farmasimed (JFM) 2025;8:220–7. https://doi.org/10.35451/7mw45p98.

[34] de Mezer M, Dolata N, Markowska J, Krzyżaniak M, Naskręt-Grochowalska A, Żurawski J, et al. Immunohistochemical expression of IL-1β, IL-6, and NF-κβ in fibroids. Front Immunol 2025;16. https://doi.org/10.3389/fimmu.2025.1571585.