The Effect of Administering Ethanol Extract of Tempuyung Leaves(Sonchus arvensis L.) on Decreasing Uric Acid LevelsIn Mile Mice

Novidawati Boru Situmorang; Azahra Salsabila Mahriza

doi:10.35451/ak6ez231

Authors

Novidawati Boru Situmorang Institut Kesehatan Medistra Lubuk Pakam
Azahra Salsabila Mahriza Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/ak6ez231

Keywords:

Tempuyung leaves (Sonchus arvensis L.), Antihyperuricemia, Male Mice (Mus musculus)

Abstract

Background: When serum uric acid levels are higher than normal, this condition is known as hyperuricemia. This condition is caused by increased breakdown of nucleic acids, decreased excretion, increased production of uric acid from purine-rich foods, or a combination of these. In traditional Indonesian medicine, tempuyung leaves (Sonchus arvensis L.) have traditionally been used to treat various diseases, including gout and kidney stones. Methods: Antihyperuricemic activity tests were conducted on 24 mice divided into six treatment groups. Hyperuricemia was induced by oral administration of chicken liver juice at a rate of 0.5 mL/20 gBW. Results: From days 1-6 after induction, uric acid levels in the test group increased progressively. In addition, the groups treated with allopurinol, 0.5% Na-CMC, and ethanol extract of tempuyung leaves (EEDT) at doses of 100, 300, and 500 mg/kgBW showed a decrease in uric acid levels on days 7 to 13. At doses of 500 mg/kgBW, 300 mg/kgBW, and 100 mg/kgBW, the allopurinol group experienced the greatest reduction, followed by the EEDT group. Conclusion: After allopurinol, the 500 mg/kgBW dose had the best antihyperuricemic efficacy, based on the percentage reduction in uric acid levels. Because the extract contains a large number of secondary metabolites, increasing the extract dose decreased the increase in effectiveness.

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References

[1] Latief, M. et al. (2021) ‘Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan’, Pharmacon: Jurnal Farmasi Indonesia, 18(1), pp. 23–37.

[2] Cendrianti, F., Muslichah, S. and Ulfa, E.L. (2023) ‘Uji Aktivitas Antihiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Daun Tempuyung (Sonchus arvensis L.) pada Mencit Jantan Hiperurisemia (Antihyperuricemic activity of n-Hexane, Ethyl acetate, and Ethanol 70% of TempuyungLeaves (Sonchus arvens’, Artikel Ilmiah Hasil Penelitian Mahasiswa, pp. 3–7.

[3] Hidayah, N. and Dalimunthe, G.I. (2022) ‘Evaluation of the Antihyperuricemia Activity of Bajakah Tampala Stem Ethanol Extract (Spatholobus littoralis Hassk.) in Male White Mice (Mus musculus)’, NSMRJ: Nusantara Scientific Medical Research Journal, 1(1), pp. 80–89.

[4] Ardiyanto, D. et al. (2021) ‘The Use of yperuricemia heHrbs at “Hortus Medicus” Herbal Medicine Clinic Tawangmangu’, Jurnal Kedokteran dan Kesehatan Indonesia. pp. 7–15

[5] Di, A., Arda, A. and Sukodono, F. (2024) ‘Profil Pengetahuan Pasien Tentang PEenggunaan Allopurinol DI Apotek Arda Frma Sukondo’, 3(1), pp. 37–45.

[6] Amal, S. et al. (2021) ‘Uji Aktivitas Antihiperurisemia Ekstrak Etanol beberapa Tumbuhan Famili Asteraceae’,Majalah Farmasetika, 6(Suppl 1), pp. 32-35.

[7] Hesty Setiawati, Nurfitriana Hayim, Jumain, H.S. (2021) ‘Aktivitas antibakteri ekstrak etanol daun kemangi (’, 6(2), pp. 6–9.

[8] Sadiah, S., Mawar Subangkit and Jurnila Sari Tanjung (2022) ‘Efektivitas Kombinasi Jus Hati Ayam Dan Serbuk Biji Melinjo Sebagai Bahan Penginduksi Hiperurisemia Pada Tikus’, Jurnal Ilmiah Manuntung, 8(1), pp. 136–144. Available at: https://doi.org/10.51352/jim.v8i1.515.

[9] Yunita, Y., Santoso, A. and Subandi, S. (2021) ‘Xanthine Oxidase Inhibitory Activity and Identification of Flavonoid in Ethanol Extract of Sugar Apple Fruit (Annona squamosa L.)’, AIP Conference Proceedings, 2353.

[10] Firawati, F., Anggrainy, H. and Hasrida, H. (2024) ‘Karakterisasi dan Identifikasi Senyawa Tanin Ekstrak Daun Tempuyung ( Sonchus arvensis L .) dan Formulasinya dalam Sediaan Losion’, Jurnal Pendidikandan Teknologi Kesehatan, 7(2), pp. 172–181.

[11] Kementrian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia (II).

[12] Nurdyansyah, F., Widyastuti, D.A. and Mandasari, A.A. (2019) ‘Karakteristik Simplisia dan Ekstrak Etanol Kulit Petai (Parkia speciosa) dengan Metode Maserasi’, Seminar Nasional Sains dan Enterpreneurship VI, pp. 1–6.

[13] Darvid, N.A., Khuluq, H. and Rahayu, T.P. (2023) ‘Uji Antidiare Ekstrak Metanol dan Ekstrak Akuades Daun Pepaya (Carica papaya L.) terhadap Tikus Galur Wistar yang Diinduksi Oleum ricini’, Journal Borneo, 3(3), pp. 149–157.

[14] Wikantyasning, E.R. et al. (2020) ‘Antihyperuricemic Effectiveness Test of Ethanol Extract of Kencur (Kaempferia galangaL.) and Black Ginger (Kaempferia parviflora) on ats Induced by hicken Liver Juice and Potassium Oxonate’, Jps, 2024(3), pp. 348–357.

[15] Harlis, W.O. and Ahmad, S.W. (2020) ‘Kadar Asam Urat Mencit Hiperurisemia Setelah Pemberian Perasan Daun Kersen ( Muntingia calabura L .) dan Daun Kelor ( Moringa oleifera L .)’, 7(1), pp. 1090–1096.

[16] Gunarti, N.S. et al. (2021) ‘Potensi Tanamana Daun Tempuyung (Sonchus arvensis L.) sebagai Antihiperurisemia Berdasarkan Kandungan Senyawa Aktif : Jurnal Buana Farma, 1(2), pp. 23–29.

[17] Amal, S. (2022) ‘Uji Toksisitas Akut Ekstrak Daun Tempuyung (Sonchus arvensis L.) pada Mencit Betina dengan Metode Fixid Dose’, Journal of Pharmacopolium, 5(2), pp. 190–198.

[18] Neni, S.G. and Hidayah, H. (2022) ‘Flavonoid compounds of tapak liman plant (Elephantopus scaber) as antihyperuricemia Senyawa flavonoid tanaman tapak liman (Elephantopus scaber) sebagai antihiperurisemia’, Jurnal Ilmiah Farmasi (Scientific Journal of Pharmacy) Special Edition, 4, pp. 31–36.

[19] Ayesha Siddiqua, S., & Krishna, V. (2025). Phytochemical Profiling and In Vitro Assessment of Antioxidant and Anti-Inflammatory Activities of Sonchus arvensis L. Journal of Chemical Health Risks, 1522–1534.

[20] Situmorang, N. B., Harahap, U., & Hasibuan, P. A. Z. (2023). Phytochemical Screening And Test For Reducing Uric Acid Levels In Male Rats After Administration Of Ethanol Extract Of The Herb Binara (Artemisia vulgaris L.). International Journal of Science, Technology & Management, 4(4), 916–923.

[21] Wahyuni, D. K., Rahmawati, C. T., Syukriya, A. J., Dismayanti, N., Arifin, M. B., Kharisma, V. D., Subramaniam, S., Zakariya, A. M., Saiman, M. Z., Ikram, N. K. K., Werbrouck, S. P. O., Purnobasuki, H., & Prasongsuk, S. (2025). Metabolite characterization and in vitro antiplasmodial potential assessment of Sonchus arvensis L. callus under glutamine treatment. Scientific Reports, 15(1), 1–14.