Formulation and Antibacterial Activity Evaluation of a Mouthwash Containing a Combination of Ethanolic Extracts of Basil Leaves (Ocimum sanctum L.) and Mint Leaves (Mentha piperita L.) against Streptococcus mutans

Nur Ulina M. Br. Turnip; Sunariati; Rosminar Hasibuan

doi:10.35451/phftxp74

Authors

Nur Ulina M. Br. Turnip Institut Kesehatan Medistra Lubuk Pakam
Sunariati Institut Kesehatan Medistra Lubuk Pakam
Rosminar Hasibuan Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/phftxp74

Keywords:

Basil Leaves, Mouthwash, Mint Leaves, Streptococcus mutans

Abstract

Background: Dental caries was a tooth decay characterized by the progressive destruction of enamel and dentin caused by the metabolic activity of Streptococcus mutans. The prevention of dental caries could be achieved by using mouthwash. However, many commercial mouthwashes contained alcohol, which might cause side effects with long-term use. Therefore, a natural-based mouthwash formulation using basil leaves (Ocimum sanctum L.) and mint leaves (Mentha piperita L.) was needed, as both contained secondary metabolites with potential antibacterial activity. Objective: This study aimed to determine whether the combination of basil leaf extract and mint leaf extract could be formulated as a mouthwash and to evaluate its antibacterial activity against Streptococcus mutans. Methods: This study used an experimental design. Basil and mint leaves were extracted by maceration using 70% ethanol. The extracts were formulated into mouthwash preparations with concentrations of 20%:0% (F1), 0%:20% (F2), and 20%:20% (F3). The formulations were evaluated through pH, stability, and clarity tests. Antibacterial activity was assessed using the disc diffusion method. Results: The results showed that the combination of basil and mint leaf extracts met the requirements for pH, stability, and clarity tests. The antibacterial activity test showed inhibition zone diameters of 14.9 mm (moderate), 15.8 mm (strong), and 16.35 mm (strong) for F1, F2, and F3, respectively. The positive control showed an inhibition zone of 25.2 mm (very strong), while the negative control showed no inhibition zone. Conclusion: The combination of basil and mint leaf extracts could be formulated as a mouthwash, met the required evaluation parameters, and exhibited antibacterial activity against Streptococcus mutans.

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References

[1] T. Ainan, W. O. Yuliastri, Dan M. Isrul, “Formulasi Dan Uji Aktivitas Antibakteri Sediaan Mouthwash Ekstrak Etanol Biji Pinang (Areca Catechu L) Terhadap Bakteri Streptococcus Mutans,” J. Pharm. Mandala Waluya, Vol. 4, No. 1, Hal. 17–31, 2025, Doi: 10.54883/Jpmw.V4i1.197.

[2] D. O. Z. Hulwah, J. Bobsaid, M. Ramadhani, Dan Y. Setiawati, “Efektivitas Mouthwash Berbahan Dasar Ekstrak Camellia Sinensis Dan Mentha Piperita Sebagai Antibakteri Terhadap Streptococcus Mutans,” J. Kedokt. Meditek, Vol. 28, No. 1, Hal. 30–39, 2022, Doi: 10.36452/Jkdoktmeditek.V28i1.2314.

[3] M. Yardha, E. Halimatushadyah, A. Yuliana, P. S. Farmasi, Dan F. Ilmu, “Formulasi Dan Uji Aktivitas Antibakteri Streptococcus Mutans Pada Sediaan Obat Kumur Kombinasi Ekstrak Daun Seledri (Apium Graveolens) Dan Daun Jambu Biji (Psidium Guajava) Pendahuluan Streptococcus Mutans Adalah Bakteri Patogen Gram Positif Berbentuk,” Vol. 12, No. 2, Hal. 1889–1903, 2024.

[4] A. Nofriyaldi, “Formulasi Sediaan Mouthwash Ekstrak Daun Sirsak (Annona Muricata L) Dengan Penambahan Variasi Konsentrasi Ekstrak Daun Kelor (Moringa Oleifera L) Sebagai Antibakteri Formulation Of Soursop Leaf Extract (Annona Muricata L.) Mouthwash With Varied C,” Vol. 10, No. December, Hal. 56–60, 2025, Doi: 10.18860/Jip.V10i2.32475.

[5] N. Sarifuddin, “Formulasi Dan Uji Aktivitas Antibakteri Streptococcus Mutans Dari Sedian Mouthwash Ekstrak Daun Salam,” Media Sains, Vol. 9, No. 1, Hal. 1–4, 2016.

[6] A. F. . & P. D. B. Oktaviani, “Oktaviani, A. F., & Pambudi, D. B. (2021). Formulasi Sediaan Obat Kumur Ekstrak Etanol Daun Selasih (Ocimum Basilicum L.) Sebagai Uji Aktivitas Antibakteri Streptococcus Mutans. Jurnal Ilmiah Jophus: Journal Of Pharmacy Umus, 3(01), 1-9.,” J. Pharm. Umus, Vol. 03, No. 01, Hal. 1–9, 2021.

[7] A. N. Syarifuddin, R. A. Purba, N. Boru Situmorang, Dan R. A. T. Marbun, “Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum Basilicum L.) Terhadap Bakteri Streptococcus Mutans,” J. Farm., Vol. 2, No. 2, Hal. 69–76, 2020, Doi: 10.35451/Jfm.V2i2.368.

[8] A. Putri, M. A. Nasution, M. S. Lubis, Dan H. M. Nasution, “Uji Aktivitas Antibakteri Sediaan Mouthwash Dari Ekstrak Daun Peppermint (Mentha Piperita L.) Dan Daun Jarak Cina (Jatropha Multifida L.) Terhadap Streptococcus Mutans,” J. Pharm. Sci., Vol. 8, No. 3, Hal. 1851–1865, 2025, Doi: 10.36490/Journal-Jps.Com.V8i3.917.

[9] N. Hidayati, E. N. Sari, H. Budiman, Dan S. Handayani, “Uji Efektivitas Antibakteri Pasta Gigi Ekstrak Daun Peppermint (Mentha Piperita L) Dan Ekstrak Daun Sirih Merah (Piper Crocatum) Terhadap Streptococcus Mutans,” Cerata J. Ilmu Farm., Vol. 14, No. 2, Hal. 97–106, 2024, Doi: 10.61902/Cerata.V14i2.868.

[10] S. M. Nabilla Aisyah Lufthya Naya, “Pharmacine,” Vol. 02, No. September, Hal. 128–145, 2021.

[11] S. Maharani Et Al., “Skrining Fitokimia Senyawa Metabolit Sekunder Dan Standarisasi Akar Manis (Glycyrrhiza Glabra L.) Phytochemical Screening Of Secondary Metabolite Compounds And Standardization Of Liquorice Root (Glycyrrhiza Glabra L.),” J. Healthc. Technol. Med., Vol. 10, No. 1, Hal. 2615–109, 2024.

[12] L. Margata, S. Nadia, M. Pakpahan, N. Hafiza, Dan F. Journal, “Penetapan Kadar Tanin Pada Rebusan Daun Porang (Amorphophallus Muelleri Blume) Dan Daun Jambu Biji (Psidium Guajava L) Dengan Metode Spektrofotometri Uv-Vis Di Indonesia Ada Berbagai Macam Tanaman Yang Tumbuh Subur . Tanaman-Tanaman Tersebut Banyak Me,” Vol. 04, Hal. 81–90, 2024.

[13] V. V. Rosalia Dan J. Rahmawati, “Formulasi Mouthwash Dari Daun Kelor (Moringa Oleifera L.) Sebagai Antibakteri Staphylococcus Aureus Penyebab Plak Gigi Moringa Leaves (Moringa Oleifera L.) Mouthwash Formulation For Antibacterial Againts Staphylococcus Aureus As A Cause Of Dental Palaque,” Usadha J. Pharm., Vol. 2, No. 4, Hal. 459–473, 2023, [Daring]. Tersedia Pada: Https://Jsr.Lib.Ums.Ac.Id/Index.Php/Ujp

[14] C. D. Voen-Na, T. S. Wardani, Dan S. M. Wicahyo, “Formulasi Dan Uji Efektivitas Antibakteri Sediaan Mouthwash Dari Ekstrak Daun Kemangi (Ocimum X Africanum L.) Terhadap Bakteri Staphylococcus Aureus,” Jifi (Jurnal Ilm. Farm. Imelda), Vol. 8, No. 2, Hal. 162–171, 2025, Doi: 10.52943/Jifarmasi.V8i2.1856.

[15] K. Mahbub, M. Walid, F. Mutiananda, Dan ..., “Formulasi Sediaan Mouthwash Ekstrak Daun Bakau (Rhizophora Apiculata Blum),” J. …, Vol. 12, No. 3, Hal. 277–284, 2023, [Daring]. Tersedia Pada: Http://Journal2.Stikeskendal.Ac.Id/Index.Php/Far/Article/View/960

[16] Eny Wijayanti, “Jurnal Ilmiah Global Farmasi,” Jigf, Vol. 2, No. 1, Hal. 2987–4742, 2024, [Daring]. Tersedia Pada: Http://Jurnal.Iaisragen.Org/Index.Php/Jigfgambaranperilakupersonalhygienepadasantriwatidengankejadian

[17] A. F. Rahmah, M. Mursyid, H. Hurria, F. Meylany, A. Husna, Dan A. Nabilah, “Formulasi Sediaan Mouthwash Antibakteri Infusa Daun Mengkudu (Morinda Citrifolia L.) Dan Daun Serai (Cymbopogon Citrates) Terhadap Streptococcus Mutans,” J. Mandala Pharmacon Indones., Vol. 10, No. 2, Hal. 432–438, 2024, Doi: 10.35311/Jmpi.V10i2.600.

[18] S. Septiani, E. N. Dewi, Dan I. Wijayanti, “Aktivitas Antibakteri Ekstrak Lamun (Cymodocea Rotundata) Terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli (Antibacterial Activities Of Seagrass Extracts (Cymodocea Rotundata) Against Staphylococcus Aureus And Escherichia Coli),” Saintek Perikan. Indones. J. Fish. Sci. Technol., Vol. 13, No. 1, Hal. 1, 2017, Doi: 10.14710/Ijfst.13.1.1-6.

[19] D. D. Octora, R. Irwanto, Dan P. C. Simarmata, “Pemanfaatan Daun Kayu Manis (Cinnamomum Burmannii , Blume) Dan Uji Efektivitasnya Sebagai Obat Kumur Terhadap Mikrofora Mulut Utilization Of Cinnamon Leaves (Cinnamomum Burmannii , Blume) And Effectiveness Test As Mouthwash Against Oral Microbes,” No. C, Hal. 48–54, 2024.

[20] A. Saptowo Dan R. Supriningrum, “Uji Aktivitas Antibakteri Ekstrak Kulit Sekilang (Embeliaborneensis Scheff ) Terhadap Bakteri Propionibacterium Acnes Dan Staphylococcus Epidermidis,” Hal. 93–97, 2021.

[21] Y. Noviyanty, F. A. Bengkulu, A. Kesehatan, Dan H. Bangsa, “Oceana Biomedicina Journal,” Ocean. Biomed. J., Vol. 4, No. 1, Hal. 38–52, 2021.

[22] Karnirius Harefa, Barita Aritonang, Dan Ahmad Hafizullah Ritonga, “Antibacterial Activity Of Ethanol Extract Of Purple Passion Fruit Peel (Passiflora Edulis Sims) On Propionibacterium Acnes Bacterial Karnirius,” J. Multidisiplin Madani, Vol. 2, No. 6, Hal. 2743–2758, 2022.