Activity Test of 25% Purified Ethanol Extract of Areca Nut on Excision Wound Healing in Male White Rats (Rattus norvegicus)
DOI:
https://doi.org/10.35451/jfm.v9i1.3674Keywords:
Areca Nut, Ethanol Extract, Purified, Wound Healing, Excision Wound, Male White RatsAbstract
Background: Excision wounds are open tissue injuries caused by sharp objects that require a complex healing process involving inflammatory, proliferative, and remodeling phases; therefore, effective and safe therapeutic agents are needed, including natural products such as areca nut (Areca catechu L.), which contains bioactive compounds with antimicrobial, anti-inflammatory, and antioxidant properties. Objective: This study aimed to determine the effectiveness of purified 25% ethanol extract of areca nut seeds in excision wound healing in male white rats and to identify the most effective concentration. Method: A true experimental study using a randomized posttest control-only group design was conducted on 25 male Sprague-Dawley rats divided into five groups: three treatment groups receiving extract concentrations of 2.5%, 5%, and 7.5%, a positive control group receiving bioplacenton, and a negative control group receiving vaseline flavum for 14 days. Observed parameters included macroscopic wound diameter reduction and histopathological features such as epidermal regeneration, collagen fibers, fibroblast count, and vascularity. Results and Discussion: The results show that the purified 25% ethanol extract of areca nut accelerates excision wound healing, indicated by faster wound contraction, more mature collagen formation, increased fibroblast activity, and improved vascularization. The 7.5% concentration shows the most optimal healing effect, approaching the positive control. Conclusion: It is concluded that purified 25% ethanol extract of areca nut seeds effectively enhances excision wound healing and has potential as a natural topical therapeutic agent.
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Comino-Sanz IM, López-Franco MD, Castro B, Pancorbo-Hidalgo PL. The role of antioxidants on wound healing: A review of the current evidence. J Clin Med 2021;10. https://doi.org/10.3390/jcm10163558.
[2] Azis A, Bulawan L, Deniyati. Uji Aktivitas Ekstrak Etanol Biji Pinang (Areca catechu L.) Terhadap Penyembuhan Luka Sayat Kelinci (Oryctolagus cuniculus). Jurnal Kesehatan Yamasi Makassar. 2022;6:8–17.
[3] Repatri A. B, Edwin Q, Deby A. M. Uji Efektivitas Salep Ekstrak Daun Jarak Pagar (Jatropha curcas L.) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus). PHARMACON. 2020;9:284–93.
[4] Simorangkir DM, Depiana E, Sianipar M, Umaya C, Apulina G. The Effect of Laban Leaf Ethanol Extract Cream (Vitex pinnata) on Wound Healing on Male White Rats (Rattus norvegicus) Wistar Strain. JURNAL FARMASIMED (JFM). 2025;7:319–26. https://doi.org/10.35451/JFM.V7I2.2713.
[5] Pranata C, Harahap HY, Herlina H, Silitonga AJ. Effectiveness Test of a 96% Ethanolic Extract Cream of Sambung Nyawa Leaves (Gynura procumbens L.) on Incision Wounds in Male White Rats. JURNAL FARMASIMED (JFM). 2026;8:1076–83. https://doi.org/10.35451/FH42GN18.
[6] Permatasari F, Yunit M, Plumeriastuti H, Arimbi, Fikri F, Ayu Wibawati P. Effect of shrimp shell chitosan on re-epithelialization of healing processes of excision wounds in white rats (Rattus norvegicus). 2023. https://doi.org/10.1063/5.0118064.
[7] Vonna A, Nurismi R, Misrahanum. Wound Healing Activity Of Unguentum Dosage Form Of Ethanolic Extracts Of Areca catechu L. NUT IN Mus musculus albinus. Jurnal Natural. 2015;15:28–36.
[8] Humaryanto, Fathnur S K, Fairuz. Topical anti-inflammatory effect of 25% ethanol extract of areca nut (Areca catechu L.) on the diameter and histopathology of osteoarthritis rat knees. Pharmaciana. 2025;15:388–97.
[9] Rairisti A. Uji aktivitas ekstrak etanol biji pinang (Areca catechu L.) terhadap penyembuhan luka sayat pada tikus putih (Rattus norvegicus.) jantan galur wistar. Jurnal Untan. 2014;1:1–17.
[10] Fairuz F, Berty KA, Humaryanto H. The Effect of Psychotria malayana Extract on Wound Healing in Male White Rats (Rattus Novergicus) Sprague Dawley Strain. Jurnal Kedokteran Brawijaya. 2023:217–22. https://doi.org/10.21776/ub.jkb.2023.032.04.3.
[11] Dian Puspitasari A, Pramono S. Comparison Of Methods Of Producing Bee Propolis Purified Extract Based On Total Flavonoid Content Using Rutin As Standard. Traditional Medicine Journal. 2015;20:2015.
[12] Ramadhani MA, Novema AP. Aktivitas antibakteri ekstrak kasar dan terpurifikasi daun cengkeh (Syzygium aromaticum) terhadap Escherichia coli dan Staphylococcus aureus. Borobudur Pharmacy Review. 2022;2:8–14. https://doi.org/10.31603/bphr.v2i1.6934.
[13] Irwandi, Dwi Agustian Harahap. Anemia et Causa Perdarahan Saluran Makan Bagian Atas. GALENICAL: Jurnal Kedokteran Dan Kesehatan Mahasiswa Malikussaleh. 2022;1.
[14] Yulia Sari E, Mutia C, Syuhada Fitri Z, Talitha Nabila Sakhi R, Maharini I, Studi Farmasi P. Formulation of Nanoemulgel Preparation from Jernang Resin Extract (Daemonorops draco (Wild.)) for Healing Burn Wounds in Male White Rats (Rattus norvegicus). JURNAL FARMASIMED (JFM). 2025;7:371–80. https://doi.org/10.35451/JFM.V7I2.2651.
[15] Djohari M, Fernando A, Safitri A. Aktivitas Daya Hambat Ekstrak Etanol Biji Pinang (Areca catechu L.) Terhadap Isolat Bakteri Gigi (Inhibition Activity Of Ethanol Extract Of Areca Catechu L. Against Isolation Of Tooth’s Bacteria). JURNAL ILMU KEFARMASIAN INDONESIA. 2020;18:81–7.
[16] Rosa VS, Hasanah F, Siahaan DN. Testing Of The Effects Of Balakacida (Chromolaena odorata L.) Leaf Extract Ointment On Wound Healing In Guinea Pigs. JURNAL FARMASIMED (JFM). 2023;5:148–56. https://doi.org/10.35451/JFM.V5I2.1530.
[17] Kangal MKO, Regan J-P. Wound Healing. StatPearls. 2023.
[18] Mamun A Al, Shao C, Geng P, Wang S, Xiao J. Recent advances in molecular mechanisms of skin wound healing and its treatments. Front Immunol. 2024;15:1395479. https://doi.org/10.3389/FIMMU.2024.1395479.
[19] Zulkefli N, Che Zahari CNM, Sayuti NH, Kamarudin AA, Saad N, Hamezah HS, et al. Flavonoids as Potential Wound-Healing Molecules: Emphasis on Pathways Perspective. International Journal of Molecular Sciences. 2023, Vol 24, Page 4607 2023;24:4607. https://doi.org/10.3390/ijms24054607.
[20] Ladeska V, Nabela T, Efendi K. Cream Formulation of Ampelas Twig Extract (Tetracera macrophylla Wall. Ex Hook.f. & Thomson) as a Wound Healer. JURNAL FARMASIMED (JFM). 2026;8:486–97. https://doi.org/10.35451/Y0N7TX23.
[21] Yaturramadhan Harahap H, Syarifuddin S, Arum C, Cahya D, Suryani I, Sinaga KB. Test of the Anti-Inflammatory Effectiveness of Ethanol Extract Gel Preparation of Sabrang Onion Bulb (Eleutherine bulbosa) on Incisional Wounds in Wistar Rats. JURNAL FARMASIMED (JFM). 2024;7:61–7. https://doi.org/10.35451/JFM.V7I1.2317.
[22] Trisha M. A, Syamsu R, Inna M. M, Ida R, Rachmat F. S. Pengaruh Pemberian Madu Hutan, Kurma Ajwa dan Gel Bioplacenton terhadap Proses Penyembuhan Luka pada Mencit. Fakumi Medical Journal. 2024;4:447–56.
[23] Sun Y, Feng J, Hou W, Qi H, Liu Y. Comprehensive insights into areca nut: active components and omics technologies for bioactivity evaluation and quality control. Front Pharmacol. 2024;15. https://doi.org/10.3389/fphar.2024.1407212.
[24] Berry CE, Brenac C, Gonzalez CE, Kendig CB, Le T, An N, et al. Natural Compounds and Biomimetic Engineering to Influence Fibroblast Behavior in Wound Healing. International Journal of Molecular Sciences. 2024, Vol 25, Page 3274 2024;25:3274. https://doi.org/10.3390/IJMS25063274.
[25] Nova P, Achmad B, David S P. Proses Penyembuhan Luka Ditinjau Dari Aspek Mekanisme Seluler Dan Molekuler. Qanun Medika. 2019;3:31–43.
[26] Megawati S, Rangkuti SN, Hidayat I, Dewi AE. The Effect of Genjer Leaf Extract Ointment (Limnocharis flava) on Incision Wounds in Male New Zealand White rabbits. JURNAL FARMASIMED (JFM) 2026;8:999–1005. https://doi.org/10.35451/TFPM7Z77.
[27] Liu E, Gao H, Zhao YJ, Pang Y, Yao Y, Yang Z, et al. The potential application of natural products in cutaneous wound healing: A review of preclinical evidence. Front Pharmacol. 2022;13:900439. https://doi.org/10.3389/fphar.2022.900439.
[28] Farhan M, Humaryanto, Kusdiyah E, Sani Kasmadi F, Harahap H. Test of 25% Ethanol Extract of Betel Nut as a Burn Wound Healing Agent in Male White Mice. JURNAL FARMASIMED (JFM). 2025;8:438–45. https://doi.org/10.35451/ADCWGH75.
[29] Hakim AR, Nayaken PO, Alawiyah T. Pengaruh Metode Ekstraksi terhadap Kadar Alkaloid Total Ekstrak Daun Kirinyuh (Chromolaena odorata): Determination of Total Alkaloid Content of Kirinyuh Leaf Extract (Chromolaena odorata) Based on Different Extraction Methods. Indonesian Journal of Pharmacy and Natural Product. 2023;6:194–200. https://doi.org/10.35473/IJPNP.V6I02.2508.
[30] Ktari N, Gargouri W, Jlaiel L, Trabelsi I, Ben Slima S, Bardaa S, et al. Extraction, Purification, Characterization, and Wound Healing Effects of Novel Prickly Pear (Opuntiaficus-indica (L.) Mill.) Heteropolysaccharides. Pharmaceuticals 2024, Vol 17, Page 1410 2024;17:1410. https://doi.org/10.3390/ph17101410.
[31] Egi Ramadhan Sm, Humaryanto, Fathnur Sani Kasmadi, Wahyu Indah Aurora, Hanina. Potensi Ekstrak Etanol 50% Terpurifikasi Biji Pinang (Areca Catechu L.) Sebagai Penyembuh Luka Bakar. Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan. 2025;11:99–106. https://doi.org/10.51352/jim.v11i2.914.
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Copyright (c) 2026 Nicholas Christobal Pasaribu, Humaryanto Humaryanto, Fathnur Sani Kasmadi, Amelia Dwi Putri, Erny Kusdiyah
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