Formulation and In Vitro Anti-Aging Activity Testing of Nyamplung (Calophyllum inophyllum L.) Leaf Extract Cream
DOI:
https://doi.org/10.35451/jfm.v9i1.3797Keywords:
Anti-aging activity, Calophyllum inophyllum L, cream, tyrosinase, in vitroAbstract
Oxidative stress caused by high levels of free radicals is a primary trigger for premature skin aging. Nyamplung leaves (Calophyllum inophyllum L.) are rich in bioactive compounds, making them highly promising for development as natural ingredients in antioxidant and anti-aging cosmetic products. This study aimed to formulate creams using nyamplung leaf extract at concentrations of 1% and 1.5%, assess their physical characteristics, and evaluate their anti-aging potential in vitro. The laboratory study employed a stepwise Soxhlet extraction method using ethyl acetate as the solvent prior to formulating the extract into creams. Physical quality testing included organoleptic assessment, homogeneity, pH, spreadability, emulsion type, viscosity, and accelerated stability testing (cycling test). Meanwhile, anti-aging activity was evaluated in vitro by measuring tyrosinase enzyme inhibition (dopachrome method) using a UV-Vis spectrophotometer. The ethyl acetate extraction yielded 4.89%. All cream formulations met the physical quality standards for topical preparations, exhibiting homogeneity, stability during the cycling test, a pH range of 5.07–5.18, spreadability of 4.9–5 cm, an oil-in-water (O/W) emulsion type, and appropriate viscosity levels. Anti-aging efficacy tests demonstrated tyrosinase enzyme inhibition rates of 20.83% at a 1% extract concentration and 5.42% at a 1.5% concentration.and demonstrated in vitro anti-aging activity, with the 1% concentration formula exhibiting higher tyrosinase inhibition activity than the 1.5% formula.
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[1] Akmal, T., & Pratama, B. A. (2023). Formulasi Dan Evaluasi Sediaan Krim Anti-Aging Ekstrak Kulit Buah Mangga Harum Manis ( Mangifera indica L .). Journal of Pharmaceutical Sciences and Clinical Pharmacy, 01(02), 37–43.
[2] Amin, N. Y. Al, Naspiah, N., & Rusli, R. (2018). Formulasi sediaan krim antiaging berbahan aktif ekstrak buah libo (Ficus variegata blume). Mulawarman Pharmaceutical Conference, 20–21.
[3] Ani, N., Awusi, R. Y. E., & Anisa, N. (2025). Pemanfaatan Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Sebagai Antioksidan Alami dalam Formulasi Krim Anti Aging. Jurnal Sains Dan Kesehatan (J. Sains Kes.), 7(5), 369–376.
[4] Budianor, Malahayati, S., & Saputri, R. (2022). Formulasi dan uji stabilitas sediaan krim ekstrak bunga melati putih ( Jasminum Sambac L . ) sebagai anti jerawat. Journal of Pharmaceutical Care Dan Sciences, 3(1), 1–13.
[5] Fadhilah, N., Yuliastri, W. O., & Bariun, L. O. (2023). Formulasi Dan Uji Aktivitas Antioksidan Sediaan Krim Antiaging Minyak Daun Nilam ( Pogostemon cablin Benth ) Menggunakan Metode ABTS. Jurnal Pharmacia Mandala Waluya, 2(5). https://doi.org/https://doi.org/10.54883/jpmw.v2i5.16
[6] Furi, M., Alfatma, A., Dona, R., Fernando, A., Aryani, F., Utami, R., Muharni, S., Husnawati, Suhery, W. N., & Octaviani, M. (2022). Uji Inhibitor EnzimTirosinase Ekstrak Dan Fraksi Daun Kedabu (Sonneratia ovata Backer) Secara In-Vitro. Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan, 8(2), 201–214.
[7] Gao, H., Xie, T., Zhang, Y., Zhao, S., Su, L., Chen, Z., & Wang, G. (2025). Vitamin D and the aging skin : insights into oxidative stress , inflammation , and barrier function. Immunity & Ageing, 22(49).
[8] Habibah, N., Noval, Melviani, & Audina, M. (2025). Formulasi Dan Evaluasi Sediaan Acne Krim Nanopartikel Ekstrak Daun Gelinggang (Cassia alata L.) Dengan Variasi Konsentrasi Setil Alkohol Dan Tween 80. Jurnal Farmasi Malahayati, 8(1), 48–60.
[9] Hastiningsih, M. C. I. (2025). The Role Of Natural Antioxidants In Delaying Premature Skin Aging Process. Jurnal Eduhealth, 16(01), 145–154. https://doi.org/10.54209/eduhealth.v16i01
[10] Vittaya, L., Chalad, C., Janyong, S., Leesakul, N. (2025). Bioactive Potential of Calophyllum inophyllum: Phytochemical Profiles, Biological Activities, and In Silico Pharmacokinetic Predictions, 94(12), 4091-4115
[11] Papaccio, F., Arino, A. D., Caputo, S., & Bellei, B. (2022). Focus on the Contribution of Oxidative Stress in Skin Aging. Antioxidants, 11(6).
[12] Purwantoro, A. I., Jayuska, A., Am, i n, & Ilmiawan, I. (2021). Uji Aktivitas Antioksidan Dari Hasil Fraksinasi Daun Nyamplung (Calophyllum inophyllum) Dengan Metode DPPH (1, 1-Difenil-2-Pikrilhidrazil). Kedokteran Inu Nafist, 10.
[13] Putri, A. R., Suhartinah, S., & Kartika, M. (2023). Uji Aktivitas Krim Anti-Aging Ekstrak Etanol Daun Bayam Merah ( Amaranthus tricolor L .) pada Kulit Punggung Kelinci New Zealand yang dipapar Sinar UV-A. Indonesian Journal Of Pharmaceutical Education, 3(1), 1–15. https://doi.org/10.37311/ijpe.v3i1.18809
[14] Riasari, H., Fitriansyah, S. N., & Hoeriah, I. S. (2022). Perbandingan Metode Fermentasi, Ekstraksi, Dan Kepolaran Pelarut Terhadap Kadar Total Flavonoid Dan Steroid Pada Daun Sukun (Artocarpus altilis (Parkinson) Fosberg). Jurnal Sains Dan Teknologi Farmasi Indonesia, XI(1).
[15] Rumanti, R. M., Fitri, K., Kumala, R., Leny, & Hafiz, I. (2022). Formulasi Sediaan Krim Anti Aging dari Ekstrak Etanol Daun Pagoda ( Clerodendrum paniculatum L.). Majalah Farmasetika, 7(4), 288–304.
[16] SR, M. A., Wahyuningsih, S., & Suryanita. (2025). Deodorant Spray Activity Test of Lemongrass Leaf ( Cymbopogon citratus ) Essential Oil and Aluminum Chlorohydrate Against Staphylococcus epidermidis Uji Aktivitas Deodorant Spray dari Minyak Atsiri Daun Serai ( Cymbopogon citratus ) dan Aluminium Klorohid. Jurnal Ilmiah Farmasi Farmasyifa, 8(2), 8–16.
[17] Sumaiyah, & Gulo, J. T. K. (2024). Formulasi dan Uji Aktivitas Anti-aging Sediaan Hand Cream yang Mengandung Ekstrak Etanol Kulit Buah Nanas (Ananas comosus (L.) Merr.). Indonesian Journal of Pharmaceutical and Clinical Research., 07(01), 046–058. https://doi.org/https://doi.org/10.32734/idjpcr.v7i1.17790.
[18] Taufiqurrahman, M., Faizatun, & Setyahadi, S. (2022). Pengembangan Krim Ekstrak Dedak Padi (Rice Bran) dan Susu Kuda Sumbawa Sebagai Antihiperpigmentasi. Jurnal Ilmiah Kesehatan, 20(3), 126–133.
[19] Tungadi, R., Pakaya, M. S., & Ali, P. D. A. (2023). Formulasi dan Evaluasi Stabilitas Fisik Sediaan Krim Senyawa Astaxanthin. 3(1), 117–124. https://doi.org/10.37311/ijpe.v3i1.14612
[20] Wijaya, H., Novitasari, & Jubaidah, S. (2023). Perbandingan Metode Ekstraksi Terhadap Rendamen Ekstrak Daun Rambai Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1), 79–83.
[21] Yusharyahya, & Shannaz Nadia. (2021). Mekanisme Penuaan Kulit sebagai Dasar Pencegahan dan Pengobatan Kulit. EJKI, 9(2), 150–159.
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