Testing the Activity of Nanochitosan Hydrogel Containing Earthworm (Lumbricus rubellus) Extract as a Future Anti-Aging Candidate
DOI:
https://doi.org/10.35451/jfm.v9i1.3820Keywords:
Earthworm, Hydrogel, Anti-aging, Nanitosan, Physical stabilityAbstract
Background: Premature aging is a skin problem influenced by exposure to solar radiation and the formation of Reactive Oxygen Species (ROS). Accumulation of ROS can cause oxidative stress, leading to skin damage such as dryness, loss of elasticity, and wrinkles. Earthworm (Lumbricus rubellus) extract contains flavonoids, alkaloids, and tannins, which have potential antioxidant activity. Formulation in the form of a nanitosan hydrogel is expected to support the delivery of active compounds into the skin. Objective: This study aimed to analyze the antioxidant activity of Lumbricus rubellus extract and evaluate the physical characteristics and stability of nanitosan hydrogel as an anti-aging candidate. Methods: The extract was obtained by maceration using 70% ethanol. Phytochemical screening was conducted for flavonoids, alkaloids, and tannins. Antioxidant activity was evaluated using the DPPH method at concentrations of 20–100 µg/mL and determined based on the IC₅₀ value. The extract was then formulated into nanitosan hydrogel at concentrations of 2.5%, 5%, and 7.5%, followed by evaluation of organoleptic properties, homogeneity, pH, spreadability, viscosity, and irritation. Results: The extract yield was 34.3% and contained flavonoids, alkaloids, and tannins. The IC₅₀ value of the extract was 95.44 ppm, indicating strong antioxidant activity. The preparations showed good homogeneity and organoleptic stability, with a pH range of 6.6–6.8 and viscosity of 28,000–32,000 cps. Visual observation showed no apparent signs of irritation. Conclusion: Lumbricus rubellus extract demonstrated strong antioxidant activity and could be formulated into nanitosan hydrogel with good physical characteristics, indicating its potential for further development as an anti-aging preparation.
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[1] Fitriani, R., Lestari, W., & Putri, A. (2021). Potensi protein dan kolagen dalam regenerasi jaringan luka bakar: Tinjauan literatur. Jurnal Farmasi dan Sains Terapan, 8(2), 101–110.
[2] Handayani, T., Sulastri, D., & Nuraini, P. (2022). Formulasi nanogel ekstrak lidah buaya (Aloe vera) dan uji aktivitas penyembuhan luka bakar pada tikus putih. Jurnal Sains Farmasi & Klinis, 9(1), 45–54.
[3] Haryanti, R., Dewi, K., & Andini, F. (2020). Efektivitas sediaan silver sulfadiazine dalam percepatan penyembuhan luka bakar. Jurnal Kedokteran dan Kesehatan, 7(3), 221–229.
[4] Pratiwi, D., Anggraini, F., & Widya, R. (2020). Aktivitas salep madu lebah terhadap penyembuhan luka bakar pada tikus putih (Rattus norvegicus). Jurnal Penelitian Kesehatan, 12(2), 67–75.
[5] Sari, D., Lubis, H., & Fauziah, R. (2021). Gel ekstrak ikan gabus (Channa striata) sebagai terapi luka bakar: Studi in vivo pada tikus putih. Jurnal Biomedis dan Farmasi, 5(1), 33–42.
[6] Wullur VM, Datu VV, Kristin Y, Kolang F, Takahindangen CJ, Komaling AV, et al. Efektifitas nanogel ekstrak kulit pisang Goroho (Musa acuminafe L.) terhadap luka bakar pada tikus putih (Rattus novergicus). 2025;13(2):95–100.
[7] Tsai SY, Lio CF, Yao WC, Liu CP, Shih SC, Wang TYT, et al. Cost-drivers of medical expenses in burn care management. Burns [Internet]. 2020;46(4):817–24. Available from: https://doi.org/10.1016/j.burns.2020.01.004
[8] Markiewicz-Gospodarek A, Kozioł M, Tobiasz M, Baj J, Radzikowska-Büchner E, Przekora A. Burn Wound Healing: Clinical Complications, Medical Care, Treatment, and Dressing Types: The Current State of Knowledge for Clinical Practice. Int J Environ Res Public Health. 2022;19(3).
[9] Pradita EY, Wahyuni S. Synthesis of Chitosan-Alginate-Siam Orange (Citrus nobilis Lour) Extract and Its Antibacterial Activity. Indones J Chem Sci. 2023;12(1):58–69.
[10] Dewi NPDC, Rata KPP, Gresitha M Stella, Pradnaya IG. Combination of PVA Chitosan Collagen Membrane and Moringa oleifera Nano Gel for Oral Wound Healing (Literature Review). Bali Dent Sci Exhib. 2024;Jan(1):1–9.
[11] Ali A, Ali A, Rahman MA, Warsi MH, Yusuf M, Alam P. Development of Nanogel Loaded with Lidocaine for Wound-Healing: Illustration of Improved Drug Deposition and Skin Safety Analysis. Gels. 2022;8(8).
[12] Kamenova K, Radeva L, Yoncheva K, Ublekov F, Ravutsov MA, Marinova MK, et al. Functional Nanogel from Natural Substances for Delivery of Doxorubicin. Polymers (Basel). 2022;14(17):1–13.
[13] Elkomy MH, Alruwaili NK, Elmowafy M, Shalaby K, Zafar A, Ahmad N, et al. Surface-Modified Bilosomes Nanogel Bearing a Natural Plant Alkaloid for Safe Management of Rheumatoid Arthritis Inflammation. Pharmaceutics. 2022;14(3).
[14] Nurlita L, Sari WY, Ramadhan F. Studi Etnobotani Tumbuhan Berkhasiat Obat. J Farmasetis. 2023;12(4):457–72.
[15] Rosilina M, Maulana MT, Chempro S, Astuti DH. Sintesis dan Modifikasi Ukuran Partikel Nano-PCC dengan Penambahan Etilen Glikol. Chempro. 2023;3(1):45–50.
[16] Chan WS, Santobuono M, D’Amico E, Selck H. The antidepressant, sertraline, impacts growth and reproduction in the benthic deposit feeder, Tubifex tubifex. Ecotoxicol Environ Saf [Internet]. 2024;285(August):117134. Available from: https://doi.org/10.1016/j.ecoenv.2024.117134
[17] Saputra D. Tinjauan Komprehensif tentang Luka Bakar dan Penanganannya. J Sci Univ Andalas Padang [Internet]. 2023;207–18. Available from: http://journal.scientic.id/index.php/sciena/issue/view/12
[18] Idham S, Rasyid RN, Nanogel RNP. Potensi Nanogel Limbah Biji Alpukat (Persea americana Mill.) Sebagai Antioksidan Pencegah Penuaan Dini pada Kulit Wajah. J Mandala Pharmacon Indones. 2024;10(2):426–31.
[19] Dipahayu D, Kusumo GG. Formulasi dan Evaluasi Nano Partikel Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea batatas L.) Varietas Antin-3. J Sains dan Kesehat. 2021;3(6):781–5.
[20] Nnamani PO, Ugwu AA, Nnadi OH, Kenechukwu FC, Ofokansi KC, Attama AA, et al. Formulation and evaluation of transdermal nanogel for delivery of artemether. Drug Deliv Transl Res [Internet]. 2021;11(4):1655–74. Available from: https://doi.org/10.1007/s13346-021-00951-4
[21] Mahdiani H, Yazdani F, Khoramipour M, Valizadeh V, Bakhshandeh H, Dinarvand R. Preparation and Physicochemical Characterization of Hyaluronic Acid-Lysine Nanogels Containing Serratiopeptidase to Control Biofilm Formation. Sci Rep [Internet]. 2024;14(1):1–14. Available from: https://doi.org/10.1038/s41598-024-56732-9
[22] Arias-Reyes C, Soliz J, Joseph V. Mice and Rats Display Different Ventilatory, Hematological, and Metabolic Features of Acclimatization to Hypoxia. Front Physiol. 2021;12(March):647882.
[23] Laguliga JA, Erviani AE, Soekendarsi E. Test The Potency Of Jatropha Sap Jatropha curcas Linn. On The Speed Of Wound Healing Burns On The Rats Skin Rattus norvegicus. Bioma J Biol Makassar [Internet]. 2021;6(2):74–83. Available from: http://journal.unhas.ac.id/index.php/bioma.
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