Evaluation of The Effectiveness of Melastoma malabathricum L. Ethanol Leaf Extract Cream on Incision Wound Healing in Male White Mice (Mus musculus)
DOI:
https://doi.org/10.35451/jfm.v9i1.3896Keywords:
Senduduk leaves (Melastoma malabathricum L.), , cream, incision wound, wound healingAbstract
Background: Incision wounds require appropriate treatment to reduce infection risk and promote tissue repair. Melastoma malabathricum leaves contain flavonoids, tannins, saponins, alkaloids, and terpenoids that may contribute to anti-inflammatory, antioxidant, and antimicrobial effects. Objective: This study investigated the wound-healing activity of ethanolic extract cream from M. malabathricum leaves at different concentrations in mice. Methods: Twenty-five male mice were assigned to five groups: Bionect® as the positive control, cream base as the negative control, and 2.5%, 5%, and 7.5% extract cream groups. A 1.5-cm-long and 2-mm-deep incision was made on the dorsal skin and treated once daily for 14 days. Wound length was assessed on days 3, 7, 10, and 14, followed by One-Way ANOVA and LSD analysis (α = 0.05). Results: The extract contained flavonoids, alkaloids, tannins, saponins, and terpenoids. All cream preparations met the physical quality requirements and produced no skin irritation. On day 14, wound lengths were 0.43 ± 0.03 cm, 0.37 ± 0.02 cm, and 0.25 ± 0.02 cm for the 2.5%, 5%, and 7.5% groups, respectively. Healing percentages were 71.00%, 75.17%, and 83.00%, respectively, while the positive and negative controls showed 100% and 66.33%. Significant differences were observed among groups (p < 0.05). Conclusion: Ethanolic extract cream of M. malabathricum leaves promoted incision wound healing in mice, with the 7.5% concentration producing the greatest effect among the tested formulations.
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[1] Ritonga AH, Amelia F, Aritonang B, et al. Potensi Antiinflamasi dari Kombinasi Ekstrak Rimpang Kunyit ( Curcuma longa L .) dan Hidroksiapatit pada Tikus Putih Jantan ( Mus musculus ) Anti-Inflammatory Potential of Combined Turmeric Rhizome Extract ( Curcuma longa L .) and Hydroxyapatite in Male White Mice ( Mus musculus ). 835–843.
[2] Stan D, Tanase C, Avram M, et al. Wound healing applications of creams and “smart” hydrogels. Exp Dermatol 2021; 30: 1218–1232.
[3] Wintoko R, Yadika ADN. Manajemen Terkini Perawatan Luka. J Kedokt Univ Lampung 2020; 3: 183–189.
[4] Abdullah F, Tahir T, Kadar K. Metode Pencucian Luka Pada Luka Akut Dan Kronik: Literature Review Fatamorgana. J Keperawatan 2022; 14: 993–1000.
[5] Ifmaily I. Pengaruh Salep Ekstrak Biji Buah Mangga Arumanis ( Mangifera Indica L ) Terhadap Penyembuhan Luka Eksisi Secara In Vivo. J Ris ilmu Kesehat dan Keperawatan; 1.
[6] Prastika DD, Setiawan B, Saputro AL, et al. Pengaruh Kitosan Udang Secara Topikal Terhadap Kepadatan Kolagen dalam Penyembuhan Luka Eksisi pada Tikus Putih. Med Vet 2020; 3: 101–107.
[7] Manuhara YSW, Sugiharto S, Kristanti AN, et al. Antioxidant Activities, Total Phenol, Flavonoid, and Mineral Content in the Rhizome of Various Indonesian Herbal Plants. Rasayan J Chem 2022; 15: 2724–2730.
[8] Giri IMDSG, Wardani IGAAK, Suena NMDS. Peran Metabolit Sekunder Tumbuhan dalam Pembentukan Kolagen pada Kulit Tikus yang Mengalami Luka Bakar. Usadha J Integr Obat Tradis 2021; 1: 23–29.
[9] Meilina A, Nindita Y, Sunarsih ES. Uji Aktivitas Ekstrak Etanol 70% Kulit Pisang Ambon Kuning (Musa acuminata Colla) Terhadap Penyembuhan Luka Sayat Kelinci (Oryctolagus cuniculus). Am J Pathol 2022; 157: 237–47.
[10] Ulina N, Turnip MB, Purba N, et al. FORMULASI DAN UJI DAYA HAMBAT JAMUR SEDIAAN KRIM EKSTRAK ETANOL BIJI ALPUKAT ( Persea americana Mill ) TERHADAP Malassezia furfur SECARA in vitro FORMULATION AND TEST INHIBITORY EFFECT OF MUSHROOMS CREAM PREPARATION ETHANOL EXTRACT AVOCADO SEEDS ( PERSEA AMERICANA MILL ) AGAINST MALASSEZIA FURFUR IN VITRO. 6. Epub ahead of print 2023. DOI: 10.35451/jfm.v6i1.1872.
[11] WIDYA F. PENGARUH PEMBERIAN GEL EKSTRAK DAUN SENDUDUK (Melastoma malabathricum L.) TERHADAP LUKA EKSISI PADA TIKUS PUTIH JANTAN.
[12] Pranata C, Pratiwi A, Medistra K, et al. Pengaruh Pemberian Jus Apel Hijau ( Malus Domestica ) Terhadap Histopatologi Organ Pulmo Tikus Putih yang Diinduksi Asap Rokok The Effect of Giving Green Apple ( Malus Domestica ) Juice on the Histopathology of White Rat Pulmonary Organs Induced by Cigarette Smoke. 2025; 162–167.
[13] POM D. Farmakope Indonesia. Edisi IV. Jakarta: Departemen Kesehatan RI, 1995.
[14] Indriaty S, Rizikiyan Y, Hidayati NR, et al. FORMULASI DAN UJI SEDIAAN KRIM BODY SCRUB OATMEAL DAN YOGURT SEBAGAI ZAT AKTIF FORMULATION AND TEST OF BODY SCRUB CREAM PREPARATION OF OATMEAL AND YOGURT AS ACTIVE. 2023; 4: 59–68.
[15] Habibah N, Noval, Melviani, et al. Formulasi Dan Evaluasi Sediaan Acne Krim Nanopartikel Ekstrak Daun Gelinggang ( Cassia alata L .) Dengan Variasi Konsentrasi Setil Alkohol Dan Tween 80. 2025; 8: 48–60.
[16] Deniansyah, Pujiastuti A. Formulasi Dan Uji Mutu Fisik Sediaan Krim Ekstrak Daun Karamunting (Rhodomytus Tomentosa). Indones J Pharm Nat Prod 2022; 05: 1.
[17] Alkohol S, Tween DAN, Habibah N, et al. FORMULASI DAN EVALUASI SEDIAAN ACNE KRIM NANOPARTIKEL EKSTRAK DAUN GELINGGANG ( Cassia alata L .) DENGAN VARIASI KONSENTRASI FORMULATION AND EVALUATION OF ACNE CREAM PREPARATION FROM GELINGGANG LEAVES EXTRACT ( Cassia alata L .) NANOPARTICLES WITH VARIOUS CONCENTRATIONS OF CETYL ALCOHOL AND TWEEN 80. 2025; 8: 48–60.
[18] Suciati A, Marfu N, Sawitri SB. Formulasi Dan Evaluasi Sediaan Lip Balm Kombinasi Minyak Alpukat Dan Ekstrak Umbi Bit Merah (Beta vulgaris L.). J Farm Malahayati 2025; 8: 356–368.
[19] Halimatussyaadiah S, Sudarmi, Anggraeni NPDA, et al. Uji iritasi formulasi obisa herbal oil ( cengkeh , ketumbar , dan kadara ) bahan pijat bayi berbasis virgin coconut oil ( VCO ) sebagai pelarut. Intisari Sains Medis 2023; 14: 1176–1180.
[20] Haryanto, Zulkifli, Pertiwi FA, et al. Studi Perbandingan Efek Anestesi Eter dan Kloroform pada Tikus Wistar ( Rattus norvegicus ) : Analisis Waktu Induksi dan Durasi Anestesi. J Ilm Ilmu Kesehat dan Kedokt; 3.
[21] Waruwu E, Agung A, Putri A, et al. Uji Standarisasi Non Spesifik Kombinasi Ekstrak Etanol Sargassum Sp. Dan Padina Sp. Di Perairan Pantai Semawang, Bali. J Media Sains 2023; 7: 67–74.
[22] Widowati R, Handayani S, Ridho A, et al. Phytochemical Screening and Antibacterial Activities of Senggani ( Melastoma malabathricum L .) Ethanolic Extract Leaves ( Skrining Fitokimia dan Aktivitas Antibakteri dari Ekstrak Daun Senggani ( Melastoma malabathricum L .)). 2021; 26: 562–568.
[23] Carica P, Terhadap L, Colletotrichum P, et al. Jurnal Agrotek Tropika. 2025; 13: 441–449.
[24] Thalia OP. Medicinal Plants in Wound Healing : Evidence Review. 7.
[25] Application T, Red OF, Oil F. Aktivitas Penyembuhan Luka Sayat Mencit ( Mus musculus ) dengan Pemberian Topikal Minyak Buah Merah ( Pandanus conoideus Lam . ). 2025; 5: 9–15.
[26] Fadilah R, Hafy Z, Larasati V. Pengembangan Bahan Alam Dari Siput Gonggong ( Laevistrombus Sp . ) Untuk Penyembuhan Luka : Tinjauan Histologis. 2025; 10: 9089–9099.
[27] Esad M, Dimov I, Choneva M, et al. Molecular Mechanisms of Wound Healing : The Role of Medicinal Plants. 2025; 1–20.
[28] Harsa IMS. Efek Pemberian Ekstrak Daun Pegagan ( Centella Asiatica ) terhadap Penyembuhan Luka Sayat pada Tikus Putih Jantan ( Rattus Norvegicus ) Galur Wistar. J Ilm Kedokt Wijaya Kusuma 2020; 2071: 21–27.
[29] Khotimah H, Ariani N, Maimunah L. Pengaruh Ekstrak Calendula Officinalis Terhadap Lama Penyembuhan Luka Sayat Pada Tikus Putih ( Rattus Norvegicus ). J Penelit Sains dan Kesehat Avicenna 2023; 2: 14–21.
[30] Yusuf K, Basir S, Maritska Z, et al. Pengaruh Terpenoid pada Bunga Telang ( Clitoria Ternatea L .) dalam Proses Penyembuhan Luka : Tinjauan Literatur. J Integr Kesehat dan Sains 2025; 7: 27–34.
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