Effectiveness of Tretinoin Gel Preparation as an Anti-Acne Agent In Rats (Rattus Novergicus)
DOI:
https://doi.org/10.35451/jfm.v9i1.3935Keywords:
Nanogel, tretinoin, Antiacne, Rattus norvegicus, GELAbstract
Background: Acne is a chronic inflammatory disorder affecting hair follicles and manifests as comedones, papules, pustules, nodules, and cysts. Its development is associated with excessive sebum secretion, abnormal keratinization, inflammation, and microbial proliferation. Propionibacterium acnes (P. acnes), a Gram-positive anaerobic bacterium, contributes to acne development by triggering inflammatory responses. Objective: This study aimed to formulate tretinoin gel and nanogel preparations and evaluate their anti-acne effectiveness. Methods: An experimental design was employed to evaluate the anti-acne effectiveness of tretinoin gel and nanogel preparations in male rats (Rattus norvegicus). Observations were conducted at 2 × 24 hours after induction and on days 7, 14, and 21. Results: The nanogel preparation showed the highest percentage reduction in ear inflammation diameter, reaching 81.68%, compared with 71.78% for Vitacid® gel and 70.10% for conventional tretinoin gel. The average particle size of the nanogel was 38.82 nm, whereas the conventional preparation had a reported particle size of 22,451 nm. Conclusion: Tretinoin nanogel demonstrated the highest anti-acne effectiveness among the tested preparations based on the reduction in ear inflammation diameter.
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[1] Minda Sari Lubis, Asmarani, A., Rafita Yuniarti, “). The Antibacterial Activity Of Conventional Serum And Nano Face Serum From Pineapple Stem Extract (Ananas Comosus (L.) Merr) Against Staphylococcus Epidermidis,” Jurnal EduHealth, vol. 15, pp. 1149–1155, 2024.
[2] Ika Fitriani, Minda Sari Lubis, Rafita Yuniarti, “Perbandingan Efektivitas Produk Topikal Anti Jerawat Terhadap Tikus Putih Jantan (Rattus Novergicus) Secara In Vivo,” Farmasainkes Jurnal Farmasi Sains, Dan Kesehatan., vol. 2, no. 1, pp. 67–76., 2022.
[3] R. N. Rina Kurniaty, Resmila Dewi, “Uji Aktivitas Serum Wajah dari Minyak Atsiri Biji Pala(Myristicae fragrans Houtt.) sebagai Anti Acne,” Jurnal Farmasimed., vol. 8, no. 2, 2026.
[4] Bilal, M., Lubis, M. S., Yuniarti, R., & Nasution, . “Formulation Of Anti-Acne Extract Aloe Vera ( Aloe vera ( L .) Burm . f .) In Hibiting The Activity Of Propionibacterium acnes,” . Jurnal International Journal of Science and Environment., pp. 241–248, 2023.
[5] Tan, A.U., Schlosser, B.J., & Paleer, “A Riview of Diagnosis and Treatment of Acne in Adult Female Patients,” Internasional Junal of Women’s Dermatology, vol. 4, pp. 56–71., 2018.
[6] Shinde, P., Sapate, R., Shinde, S., Kadam, A., Jadhav, R., Mali, H. and Gidde, “Formulation and Optimization of Semi Herbal Anti Acne Compact Face Powder by Allium Sativum and Myristica Fragrans Extract,”Jurnal Indo American Journal of Pharmaceutical Research,, vol. 11, no. 4, pp. 1642–1649, 2021.
[7] Ariani, L. W., “). Stabilitas Fisik Nanogel Minyak Zaitun (Olea europaeae L.),” Jurnal Ilmiah Cendekia Eksakta, vol. 5, no. 2, pp. 101–108., 2021.
[8] Aminah. S, “Formulasi dan Evaluasi Sediaan Nanogel Cacing Sutera (Tubifex sp.) sebagai Agen Terapi Luka Bakar Masa Depan,” Jurnal Farmasimed, vol. 8, no. 1, 2025, doi: 10.35451/p1z1pm37.
[9] Yuniarti, S Nadia, A Alamanda, M Zubir, RA Syahputra, “Characterization, phytochemical screenings and antioxidant activity test of kratom leaf ethanol extract (Mitragyna speciosa Korth) using DPPH method,” Journal of Physics, vol. 1, 2020.
[10] Rawitri, K., Miswanda, D., Rani, Z., Ningtias, A., Wayuni, S., Sari, R, S., Puteri, A, I, “Pendampingan Pemeriksaan Kadar Gula Darah Sebagai Upaya Pencegahan Komplikasi Diabetes Melitus Tipe 2,” Jurnal Pengabdian Masyarakat., vol. 3, no. 2, pp. 283–290, 2023.
[11] Kiki Rawitri, Wahyuni, S., Rani, Z., & Ginting, “Edukasi Pemanfaatan Tanaman Obat Tradisional sebagai Upaya Peningkatan Daya Tahan Tubuh.,” Jurnal Bakti Nusantara, vol. 1, no. 3, pp. 119–128, 2024, [Online]. Available: https://doi.org/10.63763/jbn.v1i3.46
[12] Fitriani, I., Lubis, M, S., Yuniarti, R & Rahayu, Y, “Perbandingan Efektivitas Produk Topikal Anti Jerawat Terhadap Tikus Jantan (Rattus novergicus) Secara In Vivo.,” farmasainkes: Jurnal Farmasi, Sains dan kesehatan., vol. 2, no. 1, 2022.
[13] Pariser, D. M., Woolery-Lloyd, H. C., Harper, J. C., Bhatt, V., Pillai, R., & Guenin, E, “The effects of once-daily tretinoin 0.05% lotion on quality of life in patients with moderate-to-severe acne vulgaris.,” American Journal of Clinical Dermatology., vol. 18, 2020, https://doi.org/10.1007/s40257-020-00559-3.
[14] Sa’diah, S., Kosim Darusman, L., Triwahyuni, W., & Batubara, “Efektivitas Krim Anti Jerawat Kayu Secang (Caesalpinia sappan) Terhadap Propionibacterium acnes pada Kulit Kelinci (Effectiveness of Anti-Acne Cream of Sappan Wood (Caesalpinia sappan) Against Propionibacterium acnes on Rabbit Skin).,” Jurnal Ilmu Kefarmasian Indonesia, vol. 11, no. 2, pp. 175–181, 2013.
[15] Yulianti, R., Abdassah, M., Abdulah, R., & Surachman, “). Gel Kombinasi Ekstrak Daun Sirsak dan Daun Jambu Biji Sebagai Obat Anti Jerawat,” Jurnal Farmasi Indonesia.vol. 7, no. 3, pp. 183–189, 2015.
[16] Tabara, “Anti-inflammatory effects of ozenoxacin, a topical quinolone antimicrobial agent,” Journal of Antibiotics.,vol. 73, no. 4, pp. 247–254., 2020.
[17] Saraogi, G. K., Tholiya, S., Mishra, Y., Mishra, V., Albutti, A., Nayak, P., & Tambuwala, “Formulation development and evaluation of pravastatin-loaded nanogel for hyperlipidemia management. Gels,” vol. 8, no. 2, p. 81, 2022, [Online]. Available: https://doi.org/10.3390/gels8020081.
[18] L. Prakash, V., & Parida, “Characterization and rheological behavior of vitamin E nanoemulsions prepared by phase inversion composition technique,” Results Eng., vol. 18, 2023, doi: https://doi.org/10.1016/j.rineng.2023.101175.
[19] S. K. Donthi, M. R., Munnangi, S., Krishna, K. V., Saha, R. N., Singhvi, G., & Dubey, “Nanoemulgel: A novel nano carrier as a tool for topical drug delivery,” Pharmaceutics, vol. 15, no. 1, p. 164, 2023, doi: https://doi.org/10.3390/pharmaceutics15010164.
[20] Tayebi-Khorrami, V., Fadaei, M. S., Dabbaghi, M. M., Azimi, E., "Overview of the application of nanogels in wound healing". Journal of Drug Delivery Science and Technology, 112. 2025. https://doi.org/10.1016/j.jddst.2025.107268
[21] Lubis, M. S., Ridwanto, & Dewi, I. N. Aplikasi Polimer Pada Sediaan Krim Body Scrub Ekstrak Etanol Ubi Jalar Ungu (Ipomoea batatas (L.) Lam). Prosiding SainsTeKes, 1, 37–57. 2019.
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Copyright (c) 2026 Febrina Chalita, Minda Sari Lubis, Rafita Yuniarti, Kiki Rawitri
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