A Comparative Study of Metabolite Properties in Moringa oleifera Extract Prepared by NADES and Organic Solvents
DOI:
https://doi.org/10.35451/jfm.v9i1.4028Keywords:
Flavonoid, Solvent, Moringa oleife, NADES, PhenolicAbstract
Background: Moringa oleifera leaves is functional food and herbal medicine that already used thousand years for various condition. Polyphenols (phenolic and flavonoid) are the main metabolite for important roles such as antioxidant for prevention and treatment diseases. Objectives: This study used Natural Deep Eutectic Solvents (NADES) with ultrasound-assisted extraction to create an effective and sustainable extraction method for phenolics and flavonoids from Moringa oleifera. Methods: Combination of Choline chloride with Urea and Glycerol in 1: 2 molar ratios have been used for the experiment. Methanol and ethanol extract in a same condition was prepared as comparison. Total Phenolic Contents (TPC) has been evaluated using Folin-Ciocalteu reagents, whereas the Total Flavonoid Content (TFC) used colorimetric assay by Spectrophotometry Uv-Vis. For extraction, the ratio of sample to solvent was 1:20. For 50 minutes, the temperature was set to 50⁰C. Results: There were 4 sample in this study such as ethanol extract, methanol extract, NADES choline chloride-glycerol (NADES CG) extract and NADES choline chloride-urea (NADES CU) extract. Conclusions: The result showed no significant values in TFC of NADES extract and organic extract. Meanwhile, TPC of organic solvent showed the highest value than NADES extract. The pH and viscosity of the NADES have an important effect on the phenolic and flavonoid extraction result.
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1. Abdallah M, Machina F, S. I. Antibacterial activity of Moringa Oleifera methanolic leaves extracts against some Gram positive and Gram negative bacterial isolates. Microbes Infect Dis. 2020 Aug 31;0(0):0–0. doi:10.21608/mid.2020.37760.1045
2. Dhakad AK, Ikram M, Sharma S, Khan S, Pandey VV, Singh A. Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytother Res. 2019 Nov;33(11):2870–903. doi:10.1002/ptr.6475
3. Nurussakinah N, Fhitriana S, Khairani TN, Utari S. Moringa Leaf (Moringa oleifera L.) Ethanol Extract Clay Mask Formulation As Anti-Aging. J Farm JFM. 2023 Oct 31;6(1):1–8. doi:10.35451/jfm.v6i1.1193
4. Agustina H. Sediaan Salep Ekstrak Daun Kelor (Moringa oleifera Lam) Sebagai Penyembuhan Luka Bakar Topikal Pada Kelinci (Oryctolagus cuniculus). Vol. 1. 2018;1.
5. Zulfikri, Nasution PR, Ernoviya. Effectiveness of Ethanol Extract of Moringa (Moringa Oleifera L.) Leaves on Increasing Hemoglobin (Hb) Levels in White Mice. J Farm JFM. 2026 Apr 30;8(2):705–12. doi:10.35451/by8j7q85
6. Srivastava R, Bhardwaj N, Jain SK, Metya AK, Parambil JV. Unveiling the potential of acetic acid-based hydrophobic natural deep eutectic solvents for phytochemical extraction. J Mol Liq. 2024;408. Located at: Scopus. doi:10.1016/j.molliq.2024.125314
7. Abdelmoumene W, Belyagoubi-Benhammou N, Kaid Z, Dich A, Belyagoubi L. Synergistic antioxidant properties of phenolic bioactives in algerian sahara herbal remedies for cancer treatment: A focus on Moringa oleifera, Ephedra alata, Anabasis articulata, and Atriplex halimus. South Afr J Bot. 2025 Nov;186:37–48. doi:10.1016/j.sajb.2025.08.034
8. Rifkia V, Prabowo I. Pengaruh Variasi Suhu dan Waktu terhadap Rendemen dan Kadar Total Flavonoid pada Ekstraksi Daun Moringa oleifera Lam. dengan Metode Ultrasonik. Pharm J Farm Indones Pharm J Indones. 2020 Dec 31;17(2):387. doi:10.30595/pharmacy.v17i2.7752
9. Yudistirani SA, Islam MB. Metode Ekstraksi Untuk Perolehan Kandungan Flavonoid Tertinggi Dari Ekstrak Daun Kelor. Vol. 8. 2019;8(2).
10. Peng W, Wang L, Wang X, Wang Y, Wang W, Huang J, et al. Efficient polyphenol extraction from Moringa oleifera Lam. leaves using natural deep eutectic solvents: COSMO-RS screening, ANN-GA optimization and antioxidant activity evaluation. LWT. 2025 May;223:117687. doi:10.1016/j.lwt.2025.117687
11. Kharbach M, Marmouzi I, El Jemli M, Bouklouze A, Vander Heyden Y. Recent advances in untargeted and targeted approaches applied in herbal-extracts and essential-oils fingerprinting - A review. J Pharm Biomed Anal. 2020 Jan;177:112849. doi:10.1016/j.jpba.2019.112849
12. Antileo-Laurie J, Olate-Olave V, Fehrmann-Riquelme V, Anabalón-Alvarez C, Cid-Carrillo L, Campanini-Salinas J, et al. Natural Deep Eutectic Solvent (NaDES) Extraction, HPLC-DAD Analysis, and Antioxidant Activity of Chilean Ugni molinae Turcz. Fruits. Antioxidants. 2025 Oct 14;14(10):1234. doi:10.3390/antiox14101234
13. Peng W, Wang X, Wang W, Wang Y, Huang J, Zhou R, et al. Comparison, optimization and antioxidant activity of ultrasound-assisted natural deep eutectic solvents extraction and traditional method: A greener route for extraction of flavonoid from Moringa oleifera Lam. leaves. Ultrason Sonochem. 2024 Oct;109:107003. doi:10.1016/j.ultsonch.2024.107003
14. Hikmawanti NPE, Saputri FC, Yanuar A, Jantan I, Ningrum RA, Juanssilfero AB, et al. Choline chloride-urea-based natural deep eutectic solvent for highly efficient extraction of polyphenolic antioxidants from Pluchea indica (L.) Less leaves. Arab J Chem. 2024 Feb;17(2):105537. doi:10.1016/j.arabjc.2023.105537
15. Ivkovic D, Cvijetic I, Radoicic A, Stojkovic-Filipovic J, Trifkovic J, Krstic Ristivojevic M, et al. NADES-Based Extracts of Selected Medicinal Herbs as Promising Formulations for Cosmetic Usage. Processes. 2024 May 13;12(5):992. doi:10.3390/pr12050992
16. Rahman MK, Fachriyah E, Kusrini D. Ekstraksi Daun Salam Berbasis Natural Deep Eutectic Solvent dan Pemanfaatannya sebagai Antioksidan. Greensphere J Environ Chem. 2023 Jan 16;2(2):7–12. doi:10.14710/gjec.2022.16569
17. Antileo-Laurie J, Olate-Olave V, Fehrmann-Riquelme V, Anabalón-Alvarez C, Cid-Carrillo L, Campanini-Salinas J, et al. Natural Deep Eutectic Solvent (NaDES) Extraction, HPLC-DAD Analysis, and Antioxidant Activity of Chilean Ugni molinae Turcz. Fruits. Antioxidants. 2025;14(10). Located at: Scopus. doi:10.3390/antiox14101234
18. Sebbah T, Yahla I, Cunha E, Riazi A, Amorim CG, Rodriguez-Diaz JM, et al. Enhanced Extraction and Separation with HPLC-DAD of Phenolic and Flavonoid Antioxidants from Portulaca oleracea L. Leaves Using Tailored Terpenoid-Based NADES: Comparative Assessment of Antiradical and Antimicrobial Activities. Antioxidants. 2025;14(2). Located at: Scopus. doi:10.3390/antiox14020132
19. Soukaina K, Safa Z, Soukaina H, Hicham C, Bouchra C. Choline chloride-based deep eutectic solvents (NADES): Potential use as green extraction media for polyphenols from Mentha pulegium, antioxidant activity, and antifungal activity. Microchem J. 2024 Apr;199:110174. doi:10.1016/j.microc.2024.110174
20. Syarifah AN, Suryadi H, Mun’im A. Total Phenolic Content of Natural Deep Eutectic Solvent (NADES) Extraction in Comfrey (Symphytum officinale). J Aisyah J Ilmu Kesehat. 2022 Jul 25;7(3):937–40. doi:10.30604/jika.v7i3.789
21. Sakti AS, Saputri FC, Mun’im A. Optimization of choline chloride-glycerol based natural deep eutectic solvent for extraction bioactive substances from Cinnamomum burmannii barks and Caesalpinia sappan heartwoods. Heliyon. 2019 Dec;5(12):e02915. doi:10.1016/j.heliyon.2019.e02915
22. Nurlinda N, Handayani V, Rasyid FA. Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves. J Fitofarmaka Indones. 2021 Mar 4;8(3):1–4. doi:10.33096/jffi.v8i3.712
23. Angelia A, Agustina AR, Fatimah U, Oktaviyanti ND. Optimization of Flavonoid Extraction and Tyrosinase Inhibitory Activity from Rhodomyrtus tomentosa Leaves Using NADES-Based Ultrasound-Assisted Extraction. Trop J Nat Prod Res. 2026;10(2):7080–6. Located at: Scopus. doi:10.26538/tjnpr/v10i2.10
24. Mahaindran A, Meng XQ, Tee LH, Chua BL, Oh KS. A New Green Solvent: Synthesis and Characterization of Natural-Deep-Eutectic-Solvent (NADES) for Application on Aqueous-Two-Phase System (ATPS) for Extraction of Anthocyanin. Mater Sci Forum. 2023 Dec 22;1111:105–10. doi:10.4028/p-mUmd2p
25. Lakshmi Papa Rao G, Mandal A, Pal N. Choline chloride-Urea based deep eutectic Solvent: Characterization, interfacial behavior and Synergism in binary (surfactant) systems. Chem Phys. 2025 Jan;588:112496. doi:10.1016/j.chemphys.2024.112496
26. Ferreira ASD, Craveiro R, Duarte AR, Barreiros S, Cabrita EJ, Paiva A. Effect of water on the structure and dynamics of choline chloride/glycerol eutectic systems. J Mol Liq. 2021 Nov;342:117463. doi:10.1016/j.molliq.2021.117463
27. James K, Long H, O’Hara I, Williams B, Moghaddam L. Bio-based film development: Harnessing alginate and fucoidan extracted from Ascophyllum nodosum with glycerol and choline chloride-based solvent. Food Hydrocoll. 2024 Aug;153:110013. doi:10.1016/j.foodhyd.2024.110013
28. Bonacci S, Di Gioia ML, Costanzo P, Maiuolo L, Tallarico S, Nardi M. Natural Deep Eutectic Solvent as Extraction Media for the Main Phenolic Compounds from Olive Oil Processing Wastes. Antioxidants. 2020 Jun 11;9(6):513. doi:10.3390/antiox9060513
29. Ivkovic D, Cvijetic I, Radoicic A, Stojkovic-Filipovic J, Trifkovic J, Krstic Ristivojevic M, et al. NADES-Based Extracts of Selected Medicinal Herbs as Promising Formulations for Cosmetic Usage. Processes. 2024;12(5). Located at: Scopus. doi:10.3390/pr12050992
30. Wu L, Chen Z, Li S, Wang L, Zhang J. Eco-friendly and high-efficient extraction of natural antioxidants from Polygonum aviculare leaves using tailor-made deep eutectic solvents as extractants. Sep Purif Technol. 2021 May;262:118339. doi:10.1016/j.seppur.2021.118339
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