IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR

  • Vincentia Ade Rizky INKES MEDISTRA LUBUK PAKAM
  • Sa’adah Siregar Institut Kesehatan Medistra Lubuk Pakam
  • Visensius Krisdianilo Institut Kesehatan Medistra Lubuk Pakam
  • Asvia Rahayu Institut Kesehatan Medistra Lubuk Pakam
  • Suventi Syafrina Ginting Institut Kesehatan Medistra Lubuk Pakam
  • Kartini . Institut Kesehatan Medistra Lubuk Pakam
Keywords: Escherichia coli O157:H7, PCR, Culture method

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.

Downloads

Download data is not yet available.

References

Bakri Z, Hatta M & Nasrum M. (2010). DETEKSI KEBERADAAN BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR. JST Kesehatan; (5), 2:184-192

Brooks GF, Butel JS, Morse SA (2005). Mikrobiologi Kedokteran Jawetz, Melnick, dan Adelberg. Edi Nugroho (alih bahasa). Jakarta : Salemba Medika

Dutta T.K., Roychoudhury S.P., Bandyopadhyay Wani S.A., and I. Hussain. (2011). DETECTION AND CHARACTERIZATION OF SHIGA TOXIN PRODUCING ESCHERICHIA COLI (STEC) & ENTEROPATHOGENIC ESCHERICHIA COLI (EPEC) IN POULTRY BIRDS WITH DIARRHOEA. Indian J. Med. Res; 133: 541-545.

Elliott T, Worthington T, Osman H, Gill M (2013). Mikrobiologi Kedokteran & Infeksi Edisi 4. Jakarta : EGC

Hermayudi dan Ayu, P.A. (2017). Penyakit daerah tropis. Yogyakarta: 2017.

Hidayatu W., Temaja I.G.R.M., dan Fatmawati N.N.D. (2018). KARAKTERISTIK FENOTIP ISOLAT KLINIK ESCHERICHIA COLI O157:H7 PADA MEDIA SORBITOL MAC CONKEY AGAR (SMAC). J. Agric.Sci. and Biotechnol, 7 (1).

Jaipah N, Saraswati I, Hapsari R (2017). UJI EFEKTIFITAS ANTIMIKROBA EKSTRAK BIJI PEPAYA (CARICA PAPAYA L.) TERHADAP PERTUMBUHAN ESCHERICHIA COLI SECARA IN VITRO. Jurnal Kedokteran Diponegoro, ISSN : 2540 − 8844, 6 (2)

Jawetz, M. A. (2010). Mikrobiologi Kedokteran. (25 ed). (G. F. Brooks, K. C. Carroll, J. S. Butel, S. A. Morse, T. A. Mietzner, Penyunt, A. W. Nugroho, D. Ramadhani, H. Santasa, N. Yasdelita, & K. W. Nimala, penerj). New York: Mc Graw Hill.

Jayanti, D. D. (2018). DETEKSI ESCHERICHIA COLI O157 PADA BERBAGAI AIR MINUM DI KELURAHAN SEKARAN GUNUNG PATI SEMARANG. Universitas Negeri Semarang

Morin NJ, Gong Z & Li XF. 2004. REVERSE TRANSCRIPTION-MULTIPLEX PCR ASSAY FOR SIMULTANEOUS DETECTION OF ESCHERICHIA COLI O157: H7, VIBRIO CHOLERAE OI, AND SALMONELLA TYPHI. Clinical Chemistry. 50(11): 2037-2044.

Peter C.H., Councell F.T., Keys C., and Monday S.R. (2011). VIRULENCE CHARACTERIZATION OF SHIGA-TOXIGENIC ESCHERICHIA COLI ISOLATES FROM WHOLESALE PRODUCE. Appl. Environ. Microbiol. 77 (1): 343-345.

ESCHERICHIA COLI PADA PENANAMAN MENGGUNAKAN TEKNIK SENGKELIT (CALIBRATED LOOP) DAN MIKROPIPET (PIPET DILUTION METHOD) METODE SPREAD PLATE. UKMC Palembang

Sartika, Indrawani, dan Sudiarti. (2005). ANALISIS MIKROBIOLOGI ESCHERICHIA COLI O157:H7 PADA HASIL OLAHAN HEWAN SAPI DALAM PROSES PRODUKSINYA. Jurnal Makara Kesehatan, 9 (1): 23-28.

Vos, P. et al. (2009). BERGEY’S MANUAL OF SYSTEMATIC BACTERIOLOGY. 2nd edn, Springer-Verlag New York. 2nd edn. Springer-Verlag New York. doi: 10.1007/978-0-387-68489-5.

Published
2021-04-30
How to Cite
Rizky, V., Siregar, S., Krisdianilo, V., Rahayu, A., Syafrina Ginting, S., & ., K. (2021). IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR. JURNAL FARMASIMED (JFM), 3(2), 118-123. https://doi.org/10.35451/jfm.v3i2.615