Effectiveness of Serum and Whole blood for Rapid HIV Testing in Pregnant Women at Batang Beruh Community Health Center
DOI:
https://doi.org/10.35451/qtttgv96Keywords:
HIV, rapid test, serum, whole blood, ibu hamilAbstract
Human Immunodeficiency Virus (HIV) remains a global health problem, particularly among pregnant women due to the risk of mother-to-child transmission. Rapid HIV testing is widely used for early detection in primary healthcare settings. Different blood specimens such as serum and whole blood can be utilized; however, their effectiveness requires evaluation. Objective to determine the effectiveness of serum and whole blood specimens in rapid HIV testing among pregnant women at Batang Beruh Health Center. Methods: This study employed a cross-sectional analytical design involving 20 pregnant women undergoing rapid HIV testing. Examination was performed using serum and whole blood specimens. Data were analyzed using Chi-square test with a significance level of 0.05. Results: Sixteen respondents (80%) showed good examination results consisting of 8 non-reactive serum results and 8 non-reactive whole blood results. Four respondents (20%) were categorized as poor results with reactive findings in both specimen types. Chi-square analysis showed p=0.02 (<0.05), indicating significant effectiveness of serum and whole blood usage. Serum and whole blood specimens are effective for rapid HIV testing among pregnant women.
Downloads
References
[1] World Health Organization. “Global HIV programme report 2023”. Geneva: WHO; 2023.
[2] Joint United Nations Programme on HIV/AIDS (UNAIDS). “Global HIV & AIDS statistics—Fact sheet”. 2023. Geneva: UNAIDS; 2023.
[3] Kementerian Kesehatan Republik Indonesia. “Pedoman nasional pencegahan penularan HIV dari ibu ke anak”. Jakarta: Kemenkes RI; 2020.
[4] Dinas Kesehatan Provinsi Sumatera Utara. “Profil kesehatan Provinsi Sumatera Utara tahun 2023”. Medan: Dinkes Sumut; 2023.
[5] Dinas Kesehatan Kabupaten Dairi. “Laporan HIV/AIDS Kabupaten Dairi tahun 2025”. Sidikalang: Dinkes Dairi; 2025.
[6] Noviana N. “Skrining HIV dan diagnosis laboratorium HIV”. Jakarta: EGC; 2021.
[7] Maryunani A. “Pencegahan penularan HIV pada ibu hamil”. Jakarta: Trans Info Media; 2020.
[8] Katiandagho D. “Pemeriksaan HIV berbasis pelayanan kesehatan primer”. Jakarta: Salemba Medika; 2020.
[9] Nadzifah F. “Spesimen darah dalam pemeriksaan laboratorium klinik”. Yogyakarta: Nuha Medika; 2020.
[10] Khasanah U. “Hematologi klinik dasar”. Jakarta: EGC; 2020.
[11] World Health Organization. “Consolidated guidelines on HIV testing services”. Geneva: WHO; 2022.
[12] Johnson CC, et al. “Real-world HIV testing strategies”. Lancet HIV. 2022;9(5):e320-e331.
[13] Tucker JD, et al. “Scaling up HIV testing worldwide”. J Int AIDS Soc. 2021;24(S7):e25835.
[14] Fogel JM, et al. “Accuracy of HIV rapid diagnostic testing algorithms”. Clin Infect Dis. 2022;75(2):271-279.
[15] Bock P, et al. “Performance of rapid HIV diagnostic tests”. J Clin Microbiol. 2021;59(8):e00374-21.
[16] UNAIDS. “Global AIDS Update 2023”. Geneva: UNAIDS; 2023.
[17] Myer L, Phillips TK. “PMTCT progress and challenges”. Lancet. 2021;397(10286):1564-1575.
[18] Joseph Davey DL, et al. “Maternal ART and vertical transmission outcomes”. J Int AIDS Soc. 2022;25(4):e25904.
[19] Gupta S, et al. “PMTCT intervention effectiveness”. AIDS Res Ther. 2021;18(1):55.
[20] Green KE, et al. “Uptake of HIV testing among pregnant women”. AIDS Behav. 2023;27(3):875-884.
[21] Delaney KP, et al. “Detection of acute HIV infection”. Clin Infect Dis. 2022;74(6):1051-1058.
[22] Stevens W, et al. “HIV diagnostic algorithms update”. AIDS. 2021;35(12):1915-1923.
[23] Pai NP, et al. “Point-of-care testing for infectious diseases”. Nat Rev Microbiol. 2022;20(6):351-363.
[24] Drain PK, et al. “Point-of-care diagnostics impact”. Lancet Infect Dis. 2021;21(9):e234-e243.
[25] Ford N, et al. “Rapid HIV testing and treatment initiation”. AIDS. 2021;35(5):779-787.
[26] Bannick M, et al. “HIV incidence estimation methods”. Epidemiology. 2023;34(5):662-670.
[27] Witzel TC, et al. “HIV self-testing effectiveness review”. BMJ Glob Health. 2021;6:e006143.
[28] Fajardo E, et al. “Advances in HIV diagnostics”. Clin Microbiol Rev. 2022;35(2):e00205-21.
[29] Peeling RW, et al. “Diagnostic innovations for infectious diseases”. Nat Rev Microbiol. 2022;20(4):193-205.
[30] Stevens WS, et al. “Strengthening laboratory systems for HIV testing”. Clin Chim Acta. 2024;548:117-124.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Nur Afni Heryanti Octavia, Rima Dhani Puspita

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright in each article is the property of the Author.
























