Analysis Of The Result Of DNA (Deoxyribonucleic Acid) Electrophoresis On Vaginal Mucosal Swabs With Cervical Cancer Patients

Authors

  • Siti Ramadhani Institut Kesehatan Medistra Lubuk Pakam
  • Vincentia Ade Rizky Institut Kesehatan Medistra Lubuk Pakam
  • Sa'adah Siregar Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/mmj.v2i1.2371

Keywords:

Cervical Cancer, ElectrophoresisRemove Cervical Cancer, Human papilloma Virus (HPV), Electrophoresis

Abstract

Background: Cervical cancer is one of the second-highest sexually transmitted diseases in the world, accounting for 13% of the total 22% of deaths caused by non-communicable diseases. Today there have been many developments about cervical cancer diagnosis methods especially in early detection aimed at recognizing cervical cancer at an earlier stage, including molecular biology detection using PCR tools and electrophoresis. Objective: To identify the results of DNA electrophoresis in vaginal mucosal swabs with cervical cancer. Method : The research method used is experimental. Results: Based on the results of a study using a documentary gel, 2 samples of HPV DNA tape were detected, it was tested positive because there was 450 bp DNA tape and 4 samples of negative results were obtained. Conclusion: The frequency distribution of respondents from 6 samples by using electrophoresis and focumerary gel as readers detected 2 positive samples. A positive result is expressed if a result in the documentary gel is found to be a DNA band. The size of the DNA ribbon produced with the DNA marker markers is at 450 bp, according to the sample target. This shows that the DNA bands in the sample are about 450 bp in size.

References

Anam, K., Cahyadi, W., Azmi, I., Senjarini, K., & Oktarianti, R. (2021). Analisis Hasil Elektroforesis Dna Dengan Image Processing Menggunakan Metode Gaussian Filter. Ijeis (Indonesian Journal Of Electronics And Instrumentation Systems), 11(1), 37. Https://Doi.Org/10.22146/Ijeis.58268

Arifa, A. (2020). Optimasi Isolasi Dna Dan Protokol Pcr (Polymerase Chain Reaction) Untuk Identifikasi Khamir Dari Sarang Lebah Madu Apis Mellifera.

Bhatla, N., Aoki, D., Sharma, D. N., & Sankaranarayanan, R. (2019). Cancer Of The Cervix Uteri. International Journal Of Gynecology And Obstetrics, 143, 22–36. Https://Doi.Org/10.1002/Ijgo.12611

Dora, Samaria., Desmawati., Lina, Ayu., Raras, Dwinova., Fauziah, Mawaddah., Nevin, Zhasmin. (2023). Edukasi Kesehatan Vaksinasi Human Papilloma Virus Untuk Mencegah Kanker Servik Pada Siswi di Jakarta Timur. Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM).

Hanina, D., Diah, Puspita., Nurul, Faridah., Salsabila, Shafa., Salma, Anisa., Nur, Alifah., Diah, Kusumawaty. (2022). Penanda DNA: Uji halal Pada Makanan Olahan Daging Menggunakan Primer Multiplex PCR (Polymerase Chain Reaction)

Harahap, M., & Hasibuan Ennysah. (2021). Pengabdian Masyarakat Penyuluhan Tentang Kanker Mulut Rahim Di Sihitang Kota Padangsidimpuan Tahun 2021. Jurnal Kebidanan Matorkis, Vol 3(No 2).

Laili, Prima., Maria, ulfa. (2020). The Correlation of The Perception of Early Detection Cervical Cancer With Attitude To Do Visual Inspection With Acetic Acid. Jurnal Ners dan Kebidnan (Journal of Ners and Midwifery)

Mohammadreza, Abbaspour., Asieh, Sadooghi., Elham, Khodaverdi., Hossein, Shahdadi. (2024). Preparation and Evaluation of Lipid-Based sustained Release Pellets of Chlorpheniramine Maleate By The Wet Extrusion-Spheronozation Method. BioImpacts.

Nengsi Destriani, S., Maryani, D., & Himalaya, D. (2022). Inspeksi Visual Asam Asetat (Iva) Pada Wanita Usia Subur (Wus) Di Puskesmas Kemumu Tahun 2022 Factors Associated With Acetic Acid Visual Inspection Behavior In Women Of Childbearing Age At The Kemumu Health Center In 2022. In Jm (Vol. 10, Issue 2).

Nur’aini, S., Mukaromah, A. S., & Muhlisoh, S. (2019). Pengenalan Deoxyribonucleic Acid (Dna) Dengan Marker-Based Augmented Reality. Walisongo Journal Of Information Technology, 1(2), 91. Https://Doi.Org/10.21580/Wjit.2019.1.2.4531

Pranani, A., Hendrayana, A., & Januartha, K. (2019). Optimasi Deteksi Gen E6 Pada Human Papillomavirus Risiko Tinggi Dengan Polymerase Chain Reaction. Jurnal Medika Udayana, Vol 8(No 11). Https://Doi.Org/10.1155/2015/892013

Nasution, P. (2021). Faktor Yang Mempengaruhi Deteksi Kanker Serviks Dengan Metode Tes Iva. Http://Ejurnalmalahayati.Ac.Id/Index.Php/Kebidanan

Novalia, V. (2023). Kanker Serviks (Vol. 2, Issue 1).

Pra, L., Serviks, K., Pasangan, W., Subur, U., Wilayah, D., Puskesmas, K., Bungo, M., Fitrisia, C. A., Khambri, D., Utama, B. I., & Muhammad, S. (2019). Analisis Faktor-Faktor Yang Berhubungan Dengan Kejadian. In Jurnal Kesehatan Andalas (Vol. 8, Issue 4). Http://Jurnal.Fk.Unand.Ac.Id

Purba, K. A., Junitha, I. K., & Wirasiti, N. N. (2022). Kuantifikasi Dna Pada Mahasiswa Perokok Dan Bukan Perokok Di Universitas Negeri Medan Kecamatan Medan Tembung Kota Medan Provinsi Sumatera Utara. Simbiosis, 10(2), 173. Https://Doi.Org/10.24843/Jsimbiosis.2022.V10.I02.P05

Ramadhaningtyas, A. (N.D.). (2020) Hubungan Seksual Usia Dini Dapat Meningkatkan Risiko Kanker Serviks.

Ridwan Harahap Program Studi Kimia, M., & Sains Dan Teknologi Uin Ar-Raniry Banda Aceh, F. (2018). Elektroforesis: Analisis Elektronika Terhadap Biokimia Genetika. 2(1), 21–26.

Samaria, D. (2022). Edukasi Kesehatan Tentang Deteksi Dini Kanker Serviks Di Desa Cibadung, Gunung Sindur, Bogor.

Septi Arimurti, I., Kusumawati, N., Haryanto, S., Widya Dharma Husada Tangerang, Stik., Pajajaran No, J., & Selatan, T. (2020). Hubungan Pendidikan Dengan Perilaku Deteksi Dini Kanker Serviks Pada Wanita Di Kelurahan Kebon Kalapa Bogor Edu Dharma Journal. Edu Dharma Journal, 4(1). Http://Openjournal.Wdh.Ac.Id/Index.Php/Edudharma

Siti Roisah, & Dina Mulyanti. (2022). Kajian Potensi Biomarker Molekuler Untuk Deteksi Dini Kanker Serviks, Hati Dan Nasofaring Serta Prospektif Kit Diagnostiknya. Bandung Conference Series: Pharmacy, 2(2). Https://Doi.Org/10.29313/Bcsp.V2i2.4308

Zubaidah., Januar, Rico. (2020). Ketahanan Hidup Pasien Kanker Serviks Berdasarkan Stadium Kanker. Jami Medical Journal, Jurnal Kedokteran dan Kesehatan.

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Published

2024-10-31