Utilization of Kluwih Seed Starch (Artocarpus camansi) as an Alternative Growth Medium for Aspergillus niger Fungus

Authors

  • saadah siregar Institut Kesehatan Medistra Lubuk Pakam
  • HAFIKA DALIMUNTHE Institut Kesehatan Medistra Lubuk Pakam
  • VINCENTIA ADE RIZKY Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/mmj.v2i1.2372

Keywords:

Kluwih Seed Starch; Aspergillus niger; Potato Dextrose Agar (PDA); Sabouraud Dextrose Agar (SDA)

Abstract

Background: Carbohydrates in kluwih seeds have sufficient nutrients, such as potatoes and tubers, making it possible to be used as a medium for mushroom growth. The nutritional composition of breadfruit contains 25% carbohydrates, 1.5% protein, and 0.3% fat. Aspergillus niger fungi require nutrients such as carbohydrates and protein to grow. This study aims to determine whether kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger fungi. Method: This study used a pre-experimental design with laboratory observation. The study was conducted by utilizing kluwih seed starch as a medium, compared to Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Results: The results showed that kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger, with fungal colonies that grow macroscopically and microscopically almost the same as PDA and SDA media. Conclusion: Kluwih seed starch can be an alternative substitute for PDA media for the growth of Aspergillus niger.

Author Biography

saadah siregar, Institut Kesehatan Medistra Lubuk Pakam

Dosen Teknik Laboratorium Medik Konsentrasi Mikrobiologi (bakteriologi dan parasitologi)

 

References

Smith, J., & Doe, A. (2018). "Comparison of Different Growth Media for Aspergillus niger." Journal of Mycology, 25(3), 123-130.

Ahmad, M. N., & Syafi'i, R. (2017). "Utilization of Starch-Based Substrates for Fungal Cultivation." Asian Journal of Microbiology, 14(2), 45-52.

Hartini, S., & Wijaya, I. (2019). "Potato Dextrose Agar and Sabouraud Dextrose Agar in Fungal Growth Analysis: A Comparative Study." International Journal of Biological Sciences, 34(1), 27-34.

Kusuma, R., & Prasetyo, T. (2020). "Nutritional Composition and Applications of Breadfruit (Artocarpus camansi) Seeds in Biotechnology." Journal of Agricultural Science, 42(5), 215-223.

Putri, E., & Ramadhani, R. (2021). "Economic Alternatives for Fungal Growth Media: From Waste to Wealth." Indonesian Journal of Biotechnology, 39(4), 56-63.

Santoso, A., & Marbun, D. (2022). "The Effect of Alternative Growth Media on the Morphological Characteristics of Aspergillus Species." Journal of Microbial Research, 50(6), 189-196.

Wang, X., & Li, Y. (2019). "Effectiveness of Organic Substrates in Supporting Fungal Growth." Mycological Research Letters, 47(3), 89-95.

Johnson, L., & Peters, K. (2020). "Natural Carbohydrate Sources as Media for Culturing Aspergillus niger." Applied Mycology, 18(5), 233-238.

Narayanan, M., & Ramasamy, K. (2021). "Utilization of Breadfruit Starch in Biotechnological Processes." Asian Biotechnology Journal, 22(7), 323-329.

Thakur, S., & Bhatt, P. (2018). "Evaluating Fungal Growth in Cost-Effective Media Substitutes." Journal of Sustainable Biotechnology, 29(2), 75-82.

Tanaka, H., & Ishikawa, K. (2019). "The Role of Non-Conventional Growth Media in Industrial Mycology." Microbiology and Biotechnology Advances, 12(4), 128-135.

Kurniawati, A., & Wahyuni, S. (2020). "Breadfruit Seed Starch as a Potential Medium for Fungi Cultivation." Indonesian Journal of Microbial Technology, 14(8), 103-109.

Choi, E., & Kim, J. (2021). "Alternative Fungal Culture Media for Cost Reduction in Biotechnology." Mycological Studies, 36(3), 141-147.

Lee, S. Y., & Kim, D. (2019). "Assessment of Carbohydrate-Based Media in Supporting Aspergillus niger Growth." Biotechnology Progress, 40(1), 93-100.

Qureshi, A., & Siddiqui, R. (2022). "Low-Cost Culture Media for Sustainable Mycology." Journal of Applied Microbial Technology, 58(2), 212-220.

Downloads

Published

2024-10-31