Formulation and Stability Evaluation of Antioxidant Sunscreen Gel Containing Green Tea Leaf (Camellia sinensis) Extract Using the DPPH Method

Jhon Patar Sinurat; Zahara Fitria; Reh Malem Br Karo; Shofian Syarifuddin

doi:10.35451/f66vym74

Authors

Jhon Patar Sinurat Institut Kesehatan Medistra Lubuk Pakam
Zahara Fitria Institut Kesehatan Medistra Lubuk Pakam
Reh Malem Br Karo Institut Kesehatan Medistra Lubuk Pakam
Shofian Syarifuddin Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.35451/f66vym74

Keywords:

Green Tea Leaves, DPPH, Formulation, Sunscreen Gel, Antioxidant Stability Test

Abstract

Sunscreen formulations are designed to protect the skin from exposure to ultraviolet (UV) radiation; however, excessive use of synthetic ingredients may lead to adverse effects on the skin. The utilization of natural ingredients as alternatives has gained increasing attention, one of which is green tea leaves (Camellia sinensis), known for their rich antioxidant content. This study aimed to formulate and evaluate the stability and antioxidant activity of a sunscreen gel based on green tea leaf extract. This research was a laboratory-based experimental study employing a quantitative approach with a post-test only control group design. The gel formulations were prepared in four variations of extract concentration, namely 0% (F0), 5% (F1), 10% (F2), and 15% (F3). The preparations were then evaluated for physical characteristics, irritation potential, and antioxidant activity using the DPPH method. The results showed that 392 g of extract was obtained from 3.8 kg of dried simplicia, yielding a 10.32% extraction yield. Phytochemical screening indicated the presence of flavonoids, tannins, saponins, and alkaloids. All formulations exhibited a semi-solid form, good homogeneity, and stable pH values ranging from 6.0 to 6.3, which are compatible with skin pH. Furthermore, no irritation reactions were observed among the 12 volunteers. Antioxidant activity increased with higher extract concentrations, with IC₅₀ values of 28.86 ppm (F0), 22.11 ppm (F1), 21.25 ppm (F2), and 20.72 ppm (F3). In addition, the percentage of inhibition for F0–F3 also showed an increasing trend, namely 8.24–17.50%, 15.79–26.28%, 13.30–24.89%, and 14.82–26.25%, respectively. In conclusion, green tea leaf extract demonstrates strong potential as an active ingredient in sunscreen gel formulations that are safe, stable, and possess significant antioxidant activity.

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References

[1] Hailun He, A. Li, S. Li, J. Tang, L. Li, And L. Xiong, “Natural Components In Sunscreens: Topical Formulations With Sun Protection Factor (SPF),” Biomed. Pharmacother., Vol. 134, P. 111161, Feb. 2021, Doi: 10.1016/J.Biopha.2020.111161.

[2] S. Y. Ng, V. R. Eh Suk, And L. T. Gew, “Plant Polyphenols As Green Sunscreen Ingredients: A Systematic Review,” J. Cosmet. Dermatol., Vol. 21, No. 11, Pp. 5409–5444, Nov. 2022, Doi: 10.1111/Jocd.15170.

[3] L. Li, L. Chong, T. Huang, Y. Ma, Y. Li, And H. Ding, “Natural Products And Extracts From Plants As Natural UV Filters For Sunscreens: A Review,” Anim. Model. Exp. Med., Vol. 6, No. 3, Pp. 183–195, Jun. 2023, Doi: 10.1002/Ame2.12295.

[4] S. Bhattacharya And A. P. Sherje, “Development Of Resveratrol And Green Tea Sunscreen Formulation For Combined Photoprotective And Antioxidant Properties,” J. Drug Deliv. Sci. Technol., Vol. 60, P. 102000, Dec. 2020, Doi: 10.1016/J.Jddst.2020.102000.

[5] S. A. Kleist And K. A. Knoop, “Understanding The Elements Of Maternal Protection From Systemic Bacterial Infections During Early Life,” Nutrients, Vol. 12, No. 4, P. 1045, Apr. 2020, Doi: 10.3390/Nu12041045.

[6] C. Musial, A. Kuban-Jankowska, And M. Gorska-Ponikowska, “Beneficial Properties Of Green Tea Catechins,” Int. J. Mol. Sci., Vol. 21, No. 5, P. 1744, Mar. 2020, Doi: 10.3390/Ijms21051744.

[7] J. Kochman, K. Jakubczyk, J. Antoniewicz, H. Mruk, And K. Janda, “Health Benefits And Chemical Composition Of Matcha Green Tea: A Review,” Molecules, Vol. 26, No. 1, P. 85, Dec. 2020, Doi: 10.3390/Molecules26010085.

[8] M. Putri, A. Hariati, And A. A. Basir, “Sunscreen Cream Formulation From Green Tea Extract (Camellia Sinensis) And SPF Value Test,” Int. J. Health Sci. (Qassim)., Vol. 3, No. 3, Pp. 522–530, Jul. 2025, Doi: 10.59585/Ijhs.V3i3.794.

[9] Firmansyah And A. Sandistira, “Jurnal Kesehatan Yamasi Makassar,” J. Kesehat. Yamasi Makasar, Vol. 4, No. 1, Pp. 98–110, 2020.

[10] P. J. Leslie And S. Gunawan, “Uji Fitokimia Dan Perbandingan Efek Antioksidan Pada Daun Teh Hijau , Teh Hitam , Dan Teh Putih ( Camellia Sinensis ) Dengan Metode DPPH ( 2 , 2-Difenil-1- Pikrilhidrazil ),” Tarumanagara Med. J., Vol. 1, No. 2, Pp. 383–388, 2019.

[11] Z. Luo And X. Zhang, “Brassica Oleracea Extract, Glucosinlates, And Sulforaphane Promote Hair Growth In Vitro And Ex Vivo,” J. Cosmet. Dermatol., Vol. 21, No. 3, Pp. 1178–1184, Mar. 2022, Doi: 10.1111/Jocd.14180.

[12] Y. Almoshari, “Novel Hydrogels For Topical Applications: An Updated Comprehensive Review Based On Source,” Gels, Vol. 8, No. 3, P. 174, Mar. 2022, Doi: 10.3390/Gels8030174.

[13] B. Shafique, M. A. Murtaza, I. Hafiz, K. Ameer, S. Basharat, And I. A. Mohamed Ahmed, “Proteolysis And Therapeutic Potential Of Bioactive Peptides Derived From Cheddar Cheese,” Food Sci. Nutr., Vol. 11, No. 9, Pp. 4948–4963, Sep. 2023, Doi: 10.1002/Fsn3.3501.

[14] K. M. Elsherif, A. M. M. Ewlad-Ahmed, E. A. A. Alhlbad, And A. M. Iqneebir, “Phytochemical Screening, Antioxidant Capacity Measurement, And Mineral Content Determination Of Thymus Vulgaris L. Extracts,” Walisongo J. Chem., Vol. 6, No. 2, Pp. 168–180, Dec. 2023, Doi: 10.21580/Wjc.V6i2.17818.

[15] Devina Chandra, Cut Masyithah Thaib, Steven Tandiono, And Manuppak Irianto Tampubolon, “Formulasi Dan Uji Penetrasi In Vitro Sediaan Gel Sistem Fitosomekstrak Buah Asam Jawa (Tamarindus Indica L.),” J. Ris. Ilmu Kesehat. Umum Dan Farm., Vol. 2, No. 4, Pp. 157–178, 2024, Doi: 10.57213/Jrikuf.V2i4.559.

[16] V. Setiawan, S. Phangestu, A. Grace Soetikno, A. Arianti, And I. Kohar, “Rapid Screening Analysis Of Antioxidant Activities In Green Tea Products Using DPPH And FRAP,” Pharm. J. Indones., Vol. 7, No. 1, Pp. 9–14, Dec. 2021, Doi: 10.21776/Ub.Pji.2021.007.01.2.

[17] M. R. Hasan Et Al., “Antioxidant Activity Study And GC-MS Profiling Of Camellia Sinensis Linn,” Heliyon, Vol. 10, No. 1, P. E23514, Jan. 2024, Doi: 10.1016/J.Heliyon.2023.E23514.

[18] K. Valentová, “Cytoprotective Activity Of Natural And Synthetic Antioxidants,” Antioxidants, Vol. 9, No. 8, P. 713, Aug. 2020, Doi: 10.3390/Antiox9080713.

[19] N. A. Fikriana, D. Chusniasih, And A. M. Ulfa, “Uji Efektivitas Ekstrak Etanol Biji Pepaya (Carica Papaya L.) Sediaan Krim Terhadap Bakteri Propionibacterium Acnes,” J. Ilmu Kedokt. Dan Kesehat., Vol. 8, No. 3, Oct. 2021, Doi: 10.33024/Jikk.V8i3.4834.

[20] G. S. R. Illah, E. S. Rejeki, And G. E. Pramukantoro, “Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Teh Hijau (Camellia Sinensis L.) Dengan Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus Polyrhizus) Menggunakan Metode DPPH,” J. Farm. Sains Indones., vol. 7, no. 2, pp. 178–185, Dec. 2024, doi: 10.52216/jfsi.vol7no2p178-185.