SOCIALIZATION OF ANALYSIS OF TOTAL TERPENOIDS FROM MANILTOA GRANDIFLORA (A. GRAY) SCHEFF LEAVES USING TLC METHODS

Authors

  • Jhon Patar Sinurat Institut Kesehatan Medistra
  • Reh Malem br Karo Universitas Prima Indonesia
  • Rinaldo Berutu

DOI:

https://doi.org/10.35451/jpk.v1i1.738

Keywords:

Terpenoids, Maceration, Partition and TLC

Abstract

Saputangan leaves  contained terpenoids which appeared reddish brown rings when tested using Liebermann Burchard, Salkowski and cerric sulfate. Saputangan leaves were macerated, then partitioned to obtain total terpenoids using a separatory funnel while checking filtrate partition with cerric sulfate. Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. TLC analyses was performed on total terpenoids using n-hexane: acetone (80:20 v/v) solvent. The total terpenoids were proved that terpenoids have 11 terpenoids as red stain based on their Rf. Based on the results of tests conducted on participants involved in socialization, it was stated that around 98% of participants had understood terpenoid compounds.

References

Lingala, S. M., & Ghany, M. G. M. Mhs. (2016). Extraction and Analysis of Terpenes/ Terpenoids Public Access. 25(3), 289–313. https://doi.org/10.1002/cppb.20024.Extraction

Ludwiczuk, A., Skalicka-Woźniak, K., & Georgiev, M. I. (2017). Terpenoids. In Pharmacognosy: Fundamentals, Applications and Strategy. https://doi.org/10.1016/B978-0-12-802104-0.00011-1

Malik, S. K., Ahmad, M., & Khan, F. (2017). Qualitative and quantitative

estimation of terpenoid contents in some important plants of Punjab, Pakistan. Pakistan Journal of Science, 69(2), 150–154.

Namir, H., Hadzic, R., Malesevic, I., Jurcevi, M., & Starcevic, D. en. (2019). Application of thin layer chromatography for qualitative analysis of gunpowder in purpose of life prediction of ammunition. International Journal of Biosensors & Bioelectronics, 5(1), 3–12.

Nn, A. (2015). A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants, 04(03), 3–8. https://doi.org/10.4172/2167-0412.1000196

Otsuka, H. (2016). Purification by Solvent Extraction Using Partition Coefficient. Natural Products Isolation, 20, 269–273. https://doi.org/10.1385/1-59259-955-9:269

Perveen, S. (2018). Introductory Chapter: Terpenes and Terpenoids. Terpenes and Terpenoids, 1–12. https://doi.org/10.5772/intechopen.79683

Rassem, H., Nour, A., & R. M., Y. (2016). Techniques For Extraction of Essential Oils From Plants: A Review. Australian Journal of Basic and Applied Sciences, 10(16), 117–127.

Scheff, A. G., & Sinurat, J. P. (2018). Isolation of Phenolic Compound & Antioxidant from Saputangan Leaves (Maniltoa Grandiflora). 5(4), 60–65

Downloads

Published

2021-06-30